MicroRNA Intercellular Transfer and Bioelectrical Regulation of Model Multicellular Ensembles by the Gap Junction Connectivity

Javier Cervera*† , Salvador Meseguer‡, and Salvador Mafe*†

† Dept. de Termodinàmica, Facultat de Física, Universitat de València, E-46100 Burjassot, Spain

‡ Laboratory of RNA Modification and Mitochondrial Diseases, Centro de Investigación Príncipe Felipe, Valencia 46012, Spain

J. Phys. Chem. B, 2017, 121 (32), pp 7602–7613

DOI: 10.1021/acs.jpcb.7b04774

Publication Date (Web): July 17, 2017

Copyright © 2017 American Chemical Society

STIMULATOR, PUMP & COMPOSITION – CalXStars Business …

Cardiac pre-differentiation of human mesenchymal stem cells by electrostimulation – Click Here

Electrostimulation induces cardiomyocyte predifferentiation of fibroblasts – Click Here

Predifferentiated Adult Stem Cells and Matrices for Cardiac Cell Therapy – Click Here

Electrostimulated bone marrow human mesenchymal stem cells produce follistatin – Click Here

In Situ Electrostimulation Drives a Regenerative Shift in the Zone of Infarcted Myocardium – Click Here

Electrical Stimulation Technologies for Wound Healing – Click Here

Possible Local Stem Cells Activation by Microcurrent – Click Here

How Microcurrent Stimulation Produces ATP – Click Here

Improving Cardiac Cell Production through Enhancing Early Mesoderm Formation – Click Here

Electrical stimulation proven to accelerate wound healing – Click Here

Cell Therapy and Tissue Generation – Click Here

0195 : Muscular effects of electrical myostimulation in heart – Click Here

Biomimetic scaffold combined with electrical stimulation and growth factor promotes tissue engineered cardiac development.

Full Text – http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3946629/

Abstract – http://www.ncbi.nlm.nih.gov/pubmed/24240126

New therapy for heart failure may enhance body’s stem cell response at cardiovascular injury site

http://www.eurekalert.org/pub_releases/2013-02/uosf-ntf022113.php

Low current electrical stimulation upregulates cytokine expression in …
www.ncbi.nlm.nih.gov/pubmed/22006493‎ 
by L Salcedo – 2012 – Related articles
Oct 18, 2011 – SDF-1 and MCP-3 after 1 h were significantly higher than after 4 h of stimulation at both time points. CONCLUSION: Electrical stimulation for 1 h …

Cxcl12 chemokine (C-X-C motif) ligand 12 [Rattus norvegicus …
www.ncbi.nlm.nih.gov/gene/24772‎ 
Apr 29, 2013 – Electrical stimulation for 1 h significantly upregulates SDF-1 and MCP-3 … Direct anal sphincter injury results in higher levels of SDF-1 and …

Importance of the SDF-1:CXCR4 Axis in Myocardial Repair
circres.ahajournals.org/content/104/10/1133.full‎ 
by MS Penn – 2009 – Cited by 60 – Related articles
The data from this study demonstrate the importance of the SDF-1:CXCR4 axis … ofSDF-1 in the setting of ischemic cardiomyopathy has been shown to improve …

Myocardial Delivery of SDF-1 in Patients with Ischemic Heart …
circres.ahajournals.org/content/…/CIRCRESAHA.113.300902.abstract‎ 
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www.ncbi.nlm.nih.gov › Journal List › NIHPA Author Manuscripts‎
by M Cheng – 2012 – Cited by 1 – Related articles
Jan 27, 2013 – Efforts to supplement SDF-1 levels in the ischemic region may alsoimprove …. However, bone marrow progenitor cell levels were lower in c-kit ….hind-limb ischemia,176 and SDF-1 also improved cardiac function after …

Angiogenic effects of stromal cell-derived factor-1 (SDF-1/CXCL12 …
www.ncbi.nlm.nih.gov/pubmed/20206813‎
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Angiogenic effects of stromal cell-derived factor-1 (SDF-1/CXCL12) variants in …PURPOSE: Critical leg ischemia (CLI) is associated with a high morbidity and mortality. … In vivo, gastrocnemius biopsies were obtained from the lower limbs of …

Effects of moderate electrical stimulation on reactive species …
www.ncbi.nlm.nih.gov/pubmed/21898396‎
by RH Lambertucci – 2012 – Related articles
Effects of moderate electrical stimulation on reactive species production by … of a moderate electrical stimulation on superoxide and nitric oxide production by … Nitric Oxide Synthase Type II/antagonists & inhibitors; Nitric Oxide Synthase Type …

Nitric oxide synthase modulates angiogenesis in response to tissue …
www.ncbi.nlm.nih.gov/pubmed/9616228‎
by T Murohara – 1998 – Cited by 1006 – Related articles
Jun 1, 1998 – We tested the hypothesis that endothelial nitric oxide synthase (eNOS)… Angiogenesis in the ischemic hindlimb was significantly improved by .

Endothelial nitric oxide synthase is critical for ischemic remodeling …
www.pnas.org/content/102/31/10999.full.pdf‎
by J Yu – 2005 – Cited by 198 – Related articles
Aug 2, 2005 – The genetic loss of endothelial-derived nitric oxide synthase …..improved blood flow recovery at 2 and 4 weeks after ischemia (Fig. 4B).

Enhancing the Outcome of Cell Therapy for Cardiac Repair
circ.ahajournals.org/content/121/2/325.full‎
by E Chavakis – 2010 – Cited by 48 – Related articles
Indeed, local injection of SDF-1 in ischemic hind limbs increased the recruitment of …the cells for cell delivery may affect the expression levels of chemokine receptors. ….Moreover, electric stimulation influences ESC differentiation.

Functional screening identifies miRNAs inducing cardiac regeneration.
www.ncbi.nlm.nih.gov/pubmed/23222520‎
by A Eulalio – 2012 – Cited by 18 – Related articles
Dec 20, 2012 – Here we show that the exogenous administration of selectedmicroRNAs (miRNAs) markedly stimulates cardiomyocyte proliferation and

A micelle-shedding thermosensitive hydrogel as sustained release …
www.ncbi.nlm.nih.gov/pubmed/22971272‎
by AJ de Graaf – 2012 – Related articles
Aug 21, 2012 – Utrecht Institute for Pharmaceutical Sciences, Pharmaceutics, Utrecht… In this paper it is shown that when a thermosensitive hydrogel based …
Utrecht University – Technology Transfer – Collective IP
https://www.collectiveip.com/technology…/utrecht…/patents?q…‎
Collective IP has compiled all of Utrecht University’s technology transfer data. Search… The invention relates to hydrogel compositions, which can be applied as …

Effective Cardiac Myocyte Differentiation of Human Induced …
www.plosone.org/article/info:doi/10.1371/journal.pone.0053764‎
by L Ye – 2013 – Related articles
Induced pluripotent stem cells (iPSCs), cells that can differentiate into all cell types… that cardiac progenitors transplanted into hearts with MI can repair the damaged …..Reinecke H, Murry CE (2002) Taking the death toll after cardiomyocyte …

Cardiomyocyte Formation by Skeletal Muscle-Derived Multi …
www.ncbi.nlm.nih.gov › Journal List › PLoS ONE › v.3(3); 2008‎
by T Tamaki – 2008 – Cited by 33 – Related articles
Mar 12, 2008 – Tetsuro Tamaki,1,2* Akira Akatsuka,1,3 Yoshinori Okada,1,3 Yoshiyasu … previously, also give rise to cardiac muscle cells as multi-myogenic stem cells, … We first identified myogenic-vasculogenic progenitor cells in the interstitial …These findings suggest that Sk-34 cells are immature stem cells that have …

Does the Human Skeletal Muscle Harbor the Murine Equivalents of …
www.ncbi.nlm.nih.gov › Journal List › Mol Ther › v.17(4); Apr 2009‎
by S Proksch – 2009 – Cited by 5 – Related articles
Autologous skeletal myoblasts have been the first cells to enter the clinical …. The most promising candidate marker to select putative cardiac progenitor …… [PubMed];Tamaki T, Akatsuka A, Ando K, Nakamura Y, Matsuzawa H, Hotta T, et al.

Isolation and expansion of adult cardiac stem cells from human and …
www.ncbi.nlm.nih.gov/pubmed/15472116‎
by E Messina – 2004 – Cited by 853 – Related articles
Isolation and expansion of adult cardiac stem cells from human and murine heart. Messina E, De Angelis L, Frati G, Morrone S, Chimenti S, Fiordaliso F, Salio M, .

Resident cardiac stem cells.
www.ncbi.nlm.nih.gov/pubmed/22114897‎
by C Frati – 2011 – Cited by 8 – Related articles
Resident cardiac stem cells. Frati C, Savi M, Graiani G, Lagrasta C, Cavalli S, Prezioso L, Rossetti P, Mangiaracina C, Ferraro F, Madeddu D, Musso E, Stilli D, ..

Cardiac resident progenitor cells: evidence and functional significance
eurheartj.oxfordjournals.org/content/early/2012/…/eurheartj.ehs208.full‎
by K Guan – 2012 – Cited by 1 – Related articles
Jul 10, 2012 – Regenerative stem cells may be derived from extracardiac sources as well as from the heart itself. … What do we know about cardiac resident progenitor cells? ….. Messina E,; De Angelis L,; Frati G,; Morrone S,; Chimenti S, …

May 25, 2012
Gladstone Scientist Receives $6.3M Award from the California Institute for Regenerative Medicine
The California Institute for Regenerative Medicine (CIRM) has awarded Deepak Srivastava, MD—who directs cardiovascular and stem cell research at the Gladstone Institutes—a $6.3 million Early Translational Award to promote stem cell-based innovation. Dr. Srivastava will use these funds to evaluate new therapies for regenerating damaged heart muscle in a preclinical setting that could lay the foundation for human clinical trials.
April 18, 2012
Gladstone Scientists Regenerate Damaged Hearts By Transforming Scar Tissue into Beating Heart Muscle
Scientists at the Gladstone Institutes today are announcing a research breakthrough in mice that one day may help doctors restore hearts damaged by heart attacks—by converting scar-forming cardiac cells into beating heart muscle.

Electrical stimulation of partial limb regeneration in mammals.
www.ncbi.nlm.nih.gov/pubmed/4503923‎ 
by RO Becker – 1972 – Cited by 77 – Related articles
Bull N Y Acad Med. 1972 May;48(4):627-41. Electrical stimulation of partial limb regeneration in mammals. Becker RO, Spadaro JA. PMCID: PMC1806700 …

Electric switch could turn on limb regeneration : Nature News
www.nature.com/uidfinder/10.1038/news070226-8‎ 
Feb 28, 2007 – Electric switch could turn on limb regeneration … some researchers hope for new approaches to stimulating tissue regeneration in humans1

Combined autologous cellular cardiomyoplasty with skeletal …
www.ncbi.nlm.nih.gov/pubmed/16153908‎ 
by IA Memon – 2005 – Cited by 53 – Related articles
Combined autologous cellular cardiomyoplasty with skeletal myoblasts and bone marrow cells in canine hearts for ischemic cardiomyopathy. Memon IA, Sawa Y …
Combined transplantation of skeletal myoblasts and bone marrow …
www.ncbi.nlm.nih.gov/pubmed/15037282‎ 
by HC Ott – 2004 – Cited by 52 – Related articles
OBJECTIVES: To prove whether intramyocardial transplantation of combined skeletalmyoblasts (SM) and mononuclear bone marrow stem cells is superior to …
Combination Cell Therapy for Heart Failure
Full Text – Circulation
circ.ahajournals.org/content/114/1_suppl/I-120.full‎ 
by LC Guarita-Souza – 2006 – Cited by 58 – Related articles
Flow cytometric analysis was performed to identify bone marrow origin stem cells ….The idea of using a combination of skeletal myoblasts, which recolonize the ….Oliveira AS, Brofman P. Cell transplantation: differential effects of myoblasts …
Neoangiogenesis after combined transplantation of skeletal …
icvts.oxfordjournals.org/content/7/2/249.full.pdf‎ 
by N Bonaros – 2008 – Cited by 21 – Related articles
Objectives: We previously reported that combined transplantation of skeletalmyoblasts and AC-133q cells … tion of skeletal myoblasts (SM) and bone marrow-derived ….. Brofman P. Simultaneous autologous transplantation of cocultured.

Myogenic specification of side population cells in skeletal muscle.
Asakura A, Seale P, Girgis-Gabardo A, Rudnicki MA.
Source
Molecular Medicine Program, Ottawa Health Research Institute, Ottawa, Ontario, K1H 8L6 Canada.
http://www.ncbi.nlm.nih.gov/pubmed/12379804

http://www.rmutrecht.org/
RMU: Regenerative Medicine Utrecht – Homepage
www.rmutrecht.org/‎ 
Regenerative Medicine (RM) is a rapidly expanding new field that brings together fundamental and clinical scientists from multiple disciplines with the aim of ..

RMU: Regenerative Medicine Utrecht – Pieter Doevendans, MD, PhD
rmutrecht.org/research/facilities/index.php?option=com…id…‎ 
Regenerative Medicine (RM) is a rapidly expanding new field that brings together fundamental and clinical scientists from multiple disciplines with the aim of .

Phase II Study Myoblasts (Muscle Stem Cells) Heart Failure 
Dr. Eric Duckers – The Netherlands 
Click Here Slide Presentation > Control – SBHCI
sbhci.org.br/wp-content/uploads/2011/03/SEISMIC_Serruys.ppt‎
Autologous Skeletal Myoblast Transplantation. in Congestive Heart Failure Patients: The SEISMIC Trial. Patrick W. Serruys, MD PhD. Eric J Duckers, MD PhD …

In situ electrostimulation drives a regenerative shift in the zone of infarcted myocardium.

Angiogenic gene-modified myoblasts promote vascularization during repair of skeletal muscle defects

Sdf-1 (CXCL12) improves skeletal muscle regeneration via the mobilisation of Cxcr4 and CD34 expressing cells.

Functional regeneration of ischemic myocardium by transplanted cells overexpressing stromal cell-derived factor-1 (SDF-1): intramyocardial injection versus scaffold-based application.

Neoangiogenesis after combined transplantation of skeletal myoblasts and angiopoietic progenitors leads to increased cell engraftment and lower apoptosis rates in ischemic heart failure.

MODIFIED SKELETAL MYOBLAST THERAPY FOR CARDIAC FAILURE USING AAV SDF1

00579

The Effect of Incorporation of SDF-1α into PLGA Scaffolds on Stem Cell Recruitment and the Inflammatory Response

Protease-Resistant Stromal Cell–Derived Factor-1 for the Treatment of Experimental Peripheral Artery Disease

Efficacy of systemic administration of SDF-1 in a model of vascular insufficiency: Support for an endothelium-dependent mechanism

Repeated implantation of skeletal myoblast in a swine model of …
eurheartj.oxfordjournals.org/…/1013.full.pdf‎

www.ncbi.nlm.nih.gov/…/16936434

eurheartj.oxfordjournals.org/…/eurheartj.ehp

Stem cells heart – Howard Leonhardt Presenting 25 Yr Experience …

Intrafibrillar silicification of collagen scaffolds for sustained release of …
www.fasebj.org/content/early/2012/07/26/fj.12-210211.full.pdf‎

Electroacupuncture preconditioning reduces cerebral ischemic …
pubmedcentralcanada.ca/pmcc/articles/PMC3562247/‎

Continuous Delivery of Stromal Cell-Derived Factor-1 … – People Page
people.hofstra.edu/…/Continuous%20Delivery%20o…‎  

Differentiation of human adult cardiac stem cells exposed to …
cardiovascres.oxfordjournals.org/content/82/3/411.full.pdf‎

Searching for Prometheus: Cell therapy and … – Atomium Culture
atomiumculture.eu
Isolation and Expansion of Adult Cardiac Stem Cells From Human …
circres.ahajournals.org/content/95/9/911.full‎  
Cardiac stem cells: isolation, expansion and experimental … – Nature
www.nature.com

Molecular Therapy http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2835114/

Mild electrical stimulation with heat stimulation increase …
www.ncbi.nlm.nih.gov/…‎  

Mild Electrical Stimulation with Heat Shock Ameliorates …
www.plosone.org/…/info%3Adoi%2F10.1371%2Fjournal.po…‎  
PLOS ONE

Mild Electrical Stimulation and Heat Shock Ameliorates …
www.plosone.org/…/info%3Adoi%2F10.1371%2Fjournal.po…‎  

Mild electrical stimulation with heat stimulation increase …
www.tripdatabase.com/…/1416291-Mild-electrical-stimulation-with-heat-…‎

Hyperthermia With Mild Electrical Stimulation Protects …
diabetes.diabetesjournals.org/content/61/4/838.long

Fatiguing stimulation of one skeletal muscle triggers heat …
jeb.biologists.org/content/…/4041.f…‎  

Use of Implantable Pumps for Growth Factor Delivery – REFERENCES

1. Epidermal Growth Factor
Q2719 Hawryluk,G.W.J., Mothe,A., Wang,J., Wang,S., Tator,C., Fehlings,M.G. An In Vivo Characterization of Trophic Factor Production Following Neural Precursor Cell or Bone Marrow Stromal Cell Transplantation for Spinal Cord Injury. STEM CELLS AND DEVELOPMENT 2012; 21(-12-):2222-2238. >>> Epidermal growth factor; fibroblast growth factor, beta; platelet-derived growth factor, alpha; CSF/CNS (intrathecal); Rat; 1007D; 7 days; Animal info (adult, female, tg).
Q2380 Paliouras,G.N., Hamilton,L.K., Aumont,A., Joppe,S.E., Barnabe-Heider,F., Fernandes,K.J.L. Mammalian Target of Rapamycin Signaling Is a Key Regulator of the Transit-Amplifying Progenitor Pool in the Adult and Aging Forebrain. Journal of Neuroscience 2012; 32(-43-):15012-15026. >>> Rapamycin; epidermal growth factor; DMSO; CSF/CNS; Mice (pregnant); 1007D; 7 days; Control animals received mp w/ vehicle; animal info (C57BL/6, female, 2, 10, 18 mo old); ALZET brain infusion kit 3 used.
Q2021 Karimi-Abdolrezaee,S., Schut,D., Wang,J., Fehlings,M.G. Chondroitinase and Growth Factors Enhance Activation and Oligodendrocyte Differentiation of Endogenous Neural Precursor Cells after Spinal Cord Injury. PLoS One 2012; 7(-5-):U605-U620. >>> Epidermal growth factor; fibroblast growth factor, basic; platelet derived growth factor-AA; Saline; albumin, rat serum; CSF/CNS (intrathecal); Rat; 1007D; 7 days; Controls received mp w/ vehicle; animal info (Wistar, female, 250 g); spinal cord injury; intrathecal catheter used (0007741).
Q1565 Hewitt,S.C., Kissling,G.E., Fieselman,K.E., Jayes,F.L., Gerrish,K.E., Korach,K.S. Biological and biochemical consequences of global deletion of exon 3 from the ER-alpha gene. FASEB Journal 2010; 24(-12-):4660-4667. >>> Epidermal growth factor; Mice; 24 hours; Animal info (Ex3 alpha ERKO).
Q1335 Sun,D., Bullock,M.R., Altememi,N., Zhou,Z.W., Hagood,S., Rolfe,A., McGinn,M.J., Hamm,R., Colello,R.J. The Effect of Epidermal Growth Factor in the Injured Brain after Trauma in Rats. Journal of Neurotrauma 2010; 27(-5-):923-938. >>> Epidermal growth factor, recomb. human; CSF, artificial; CSF/CNS; Rat; 1007D; 7 days; Controls received mp w/ vehicle; animal info (male, 3-4 mo old, Sprague Dawley, 300 g); functionality of mp verified by residual volume; ALZET brain infusion kit 2 used; artificial CSF recipe; cannula placement verified by injecting Evan’s Blue dye into the cannula.
Q1151 Joseph,N.M., He,S.H., Quintana,E., Kim,Y.G., Nunez,G., Morrison,S.J. Enteric glia are multipotent in culture but primarily form glia in the adult rodent gut. Journal of Clinical Investigation 2011; 121(-9-):3398-3411. >>> Epidermal growth factor; fibroblast growth factor; glial-derived neurotrophic factor; BSA; PBS; IP; Rat; 7 days; Controls received mp w/ vehicle; animal info (P90).
Q1055 Erlandsson,A., Lin,C.H.A., Yu,F.G., Morshead,C.M. Immunosuppression promotes endogenous neural stem and progenitor cell migration and tissue regeneration after ischemic injury. Experimental Neurology 2011; 230(-1-):48-57. >>> Epidermal growth factor, recomb. human; erythropoietin; cyclosporine A; CSF/CNS; SC; Mice; 1007D; ; Animal info (male, C57/BL6, 8-10 wks old); pumps replaced after 7 days; ALZET brain infusion kit 3 used.
Q0798 Miksa,M., Wu,R.Q., Dong,W.F., Komura,H., Amin,D., Ji,Y.X., Wang,Z.M., Wang,H.C., Ravikumar,T.S., Tracey,K.J., Wang,P. Immature Dendritic Cell-Derived Exosomes Rescue Septic Animals Via Milk Fat Globule Epidermal Growth Factor VIII. Journal of Immunology 2009; 183(-9-):5983-5990. >>> Epidermal growth factor VIII, milk fat globule, recomb. murine; IV (jugular); Rat; 2001D; 20 hours; Controls received mp w/ PBS; immunology; animal info (male, Sprague-Dawley).
Q0793 Gonzalez-Perez,O., Romero-Rodriguez,R., Soriano-Navarro,M., Garcia-Verdugo,J.M., varez-Buylla,A. Epidermal Growth Factor Induces the Progeny of Subventricular Zone Type B Cells to Migrate and Differentiate into Oligodendrocytes. Stem Cells 2009; 27(-8-):2032-2043. >>> Epidermal growth factor; BSA; saline; CSF/CNS; Mice; 1007D; 7 days; Controls received mp w/ vehicle; animal info (adult, CD-1); schematic of pump and cannula placement, figure 4.
Q0699 Gampe,K., Brill,M.S., Momma,S., Goetz,M., Zimmermann,H. EGF induces CREB and ERK activation at the wall of the mouse lateral ventricles. Brain Research 2011; 1376(-;-):31-41. >>> Epidermal growth factor, recomb.; CSF, artificial; CSF/CNS; Mice; 1007D; 6 days; Controls received mp w/ vehicle; animal info (,ale, C57BL/6N, 8-10 wks old); artificial CSF formula.
Q0397 Aguirre,A., Rubio,M.E., Gallo,V. Notch and EGFR pathway interaction regulates neural stem cell number and self-renewal. Nature 2010; 467(-7313-):323-U101. >>> Ara-C; epidermal growth factor; Saline; CSF/CNS (surface); CSF/CNS; Mice; 5, 6 days; Controls received mp w/vehicle; animal info (Cnp-hEGFR); incorrectly stated model 1007.
Q0248 Kolb,B, Morshead,C., Gonzalez,C., Kim,M., Gregg,C., Shingo,T., Weiss,S. Growth factor-stimulated generation of new cortical tissue and functional recovery after stroke damage to the motor cortex of rats. Journal of Cerebral Blood Flow and Metabolism 2007; 27(–):983-997. >>> Erythropoietin; epidermal growth factor; CSF, artificial; CSF/CNS; Rat; 2001; 7, 14 days; Controls received mp w/ vehicle; peptides; animal info (male, Long-Evans, 90-110 days old); ischemia (cerebral); behavioral testing (forelimb assymetry, forelimb inhibition (swimming), reaching); some animals received 7 days EGF.
Q0241 Im,S.H., Yu,J.H., Park,E.S., Lee,J.E., Kim,H.O., Park,K.I., Kim,G.W., Park,C.I., Cho,S.R. INDUCTION OF STRIATAL NEUROGENESIS ENHANCES FUNCTIONAL RECOVERY IN AN ADULT ANIMAL MODEL OF NEONATAL HYPOXIC-ISCHEMIC BRAIN INJURY. Neuroscience 2010; 169(-1-):259-268. >>> Brain-derived neurotrophic factor; epidermal growth factor; ara-C; CSF/CNS; Mice; 1002; 2 weeks; Controls received mp w/ PBS; ALZET brain infusion kit 3 used; animal info (ICR, 6 wks old); behavioral testing (rotarod performance, forelimb-use asymmetry test).
Q0061 Karimi-Abdolrezaee,S., Eftekharpour,E., Wang,J., Schut,D., Fehlings,M.G. Synergistic Effects of Transplanted Adult Neural Stem/Progenitor Cells, Chondroitinase, and Growth Factors Promote Functional Repair and Plasticity of the Chronically Injured Spinal Cord. Journal of Neuroscience 2010; 30(-5-):1657-1676. >>> Chondroitinase ABC; epidermal growth factor; fibroblast growth factor; platelet-derived growth factor; Penicillinase; saline; albumin, rat serum; CSF, artificial; gentamicin; BSA; CSF/CNS (intrathecal, subarachnoid space); Rat; 1007D; 7 days; Controls received mp w/ vehicle; animal info (female, Wistar, 250 g.); ALZET intrathecal catheter used (0007741).
P9944 Agrawal,A., Min,D.H., Singh,N., Zhu,H.H., Birjiniuk,A., von Maltzahn,G., Harris,T.J., Xing,D.Y., Woolfenden,S.D., Sharp,P.A., Charest,A., Bhatia,S. Functional Delivery of siRNA in Mice Using Dendriworms. ACS Nano 2009; 3(-9-):2495-2504. >>> RNA, small interfering; epidermal growth factor receptor; dendriworm, dye-labeled; RNA, small interfering, GFP, dendriworm, dye-labeled; CSF/CNS (intratumoral); Mice; 3, 7 days; Tissue perfusion (tumor); cancer (glioblastoma); incorrectly stated pump model 2007; Plastics One cannula used; animial info (Swiss Webster).
P9843 Oya,S., Yoshikawa,G., Takai,K., Tanaka,J.I., Higashiyama,S., Saito,N., Kirino,T., Kawahara,N. ATTENUATION OF Notch SIGNALING PROMOTES THE DIFFERENTIATION OF NEURAL PROGENITORS INTO NEURONS IN THE HIPPOCAMPAL CA1 REGION AFTER ISCHEMIC INJURY. Neuroscience 2009; 158(-2-):683-692. >>> Epidermal growth factor, recomb. human; fibroblast growth factor, recomb. human; Jag1, recomb. rat; S2188; DLL4, recomb. mouse; CSF, artificial, DMSO; CSF/CNS; Rat; 1003D; 1007D; 11 days; Controls received mp w/ vehicle; pumps replaced after 3 days; enzyme inhibitor (gamma secretase); animal info (male, Wistar, adult, 8 wks old, 280-320 g.); ischemia (cerebral); bilateral infusion; 10% DMSO used; brain tissue distribution.
P9520 Thatch,K.A., Schwartz,M.Z., Yoo,E.Y., Mendelson,K.G., Duke,D.S. Modulation of the inflammatory response and apoptosis using epidermal growth factor and hepatocyte growth factor in a liver injury model: a potential approach to the management and treatment of cholestatic liver disease. Journal of Pediatric Surgery 2008; 43(-12-):2169-2173. >>> Epidermal growth factor, recomb. human; human growth factor, recomb. human; IV; Rat; 7 days; Controls received mp w/ PBS; animal info (female, adult, Sprague Dawley, 200-250 g.).
P9320 De Toni,A., Zbinden,M., Epstein,J.A., Altaba,A.R.I., Prochiantz,A., Caille,I. Regulation of survival in adult hippocampal and glioblastoma stem cell lineages by the homeodomain-only protein HOP. Neural Development 2008; 3(-;-):U2-U13. >>> Epidermal growth factor; fibroblast growth factor, basic; RNA, small interfering; Saline, physiological; CSF/CNS; CSF/CNS (dentate gyrus); Rat; 1003D; 3 days; Controls received mp w/ control siRNA; cancer (glioblastoma); peptides; animal info (Swiss, HOP -/-, wt, adult); HOP or control siRNA was coupled to the cell permeant peptide, penetratin.
P8932 Oya,S., Yoshikawa,G., Takai,K., Tanaka,J.I., Higashiyama,S., Saito,N., Kirino,T., Kawahara,N. Region-specific proliferative response of neural progenitors to exogenous stimulation by growth factors following ischemia. NeuroReport 2008; 19(-8-):805-810. >>> Epidermal growth factor, recomb. human; fibroblast growth factor-2, recomb. human; insulin-like growth factor I, recomb. human; erythropoietin, recomb. rat; brain-derived neurotrophic factor, recomb. human; DDL4, recomb. mouse; CSF/CNS; Rat; 1003D; 3 days; Ischemia; animal info (male, Wistar, 8wks old, 280-300 g.); bilateral infusion.
P8794 Louis,S.A., Rietze,R.L., Deleyrolle,L., Wagey,R.E., Thomas,T.E., Eaves,A.C., Reynolds,B.A. Enumeration of neural stem and progenitor cells in the neural colony-forming cell assay. Stem Cells 2008; 26(-4-):988-996. >>> Epidermal growth factor; ara-c; Saline; CSF/CNS; Mice; 1007D; 7 days; Controls received mp w/ vehicle; cyanoacrylate adhesive; animal info (adult, C57BL/6).
P8705 Mizuno,M., Sotoyama,H., Narita,E., Kawamura,H., Namba,H., Zheng,Y.J., Eda,T., Nawa,H. A cyclooxygenase-2 inhibitor ameliorates behavioral impairments induced by striatal administration of epidermal growth factor. Journal of Neuroscience 2007; 27(-38-):10116-10127. >>> Epidermal growth factor, recomb. human; Saline; CSF/CNS (striatum); CSF/CNS (nucleus accumbens); Rat; 2002; 8-14 days; Controls received mp w/ vehicle; functionality of mp verified by residual volume; dose-response (fig. 1); peptides; multiple pumps per animal (2); post op. care (cefmetazon); animal info (male, Sprague-Dawley, 7-8 wks old, 300-380g); cannula position confirmed after completion of tests.
P8632 Han,G.P., Li,L., Kosugi,I., Kawasaki,H., Tsuchida,T., Miura,K., Tsutsui,Y. Enhancement of susceptibility of adult mouse brain to cytomegalovirus infection by infusion of epidermal growth factor. Journal of Neuroscience Research 2007; 85(-13-):2981-2990. >>> Epidermal growth factor, mouse; PBS; CSF/CNS; Mice; 1003D; 3 days; Controls received mp w/ vehicle; peptides; animal info (female, BALB/c; 10, 25, and 70 wks old).
2. Fibroblast Growth Factor
Q2719 Hawryluk,G.W.J., Mothe,A., Wang,J., Wang,S., Tator,C., Fehlings,M.G. An In Vivo Characterization of Trophic Factor Production Following Neural Precursor Cell or Bone Marrow Stromal Cell Transplantation for Spinal Cord Injury. STEM CELLS AND DEVELOPMENT 2012; 21(-12-):2222-2238. >>> Epidermal growth factor; fibroblast growth factor, beta; platelet-derived growth factor, alpha; CSF/CNS (intrathecal); Rat; 1007D; 7 days; Animal info (adult, female, tg).
Q2655 Kharitonenkov,A., Beals,J.M., Micanovic,R., Strifler,B.A., Rathnachalam,R., Wroblewski,V.J., Li,S., Koester,A., Ford,A.M., Coskun,T., Dunbar,J.D., Cheng,C.C., Frye,C.C., Bumol,T.F., Moller,D.E. Rational Design of a Fibroblast Growth Factor 21-Based Clinical Candidate, LY2405319. PLoS One 2013; 8(-3-):U650-U659. >>> Fibroblast growth factor-21; SC; Mice; 7, 14 days; Control animals received mp w/ vehicle; animal info (18 wks old, DIO, C57BL/6).
Q2645 Adams,A.C., Cheng,C.C., Coskun,T., Kharitonenkov,A. FGF21 Requires betaklotho to Act In Vivo. PLoS One 2012; 7(-11-):U405-U412. >>> Fibroblast growth factor-21; Mice; 14 days; Animal info (male, wt, KLBKO).
Q2480 Murphy,M., Samms,R., Warner,A., Bolborea,M., Barrett,P., Fowler,M.J., Brameld,J.M., Tsintzas,K., Kharitonenkov,A., Adams,A.C., Coskun,T., Ebling,F.J.P. Increased Responses to the Actions of Fibroblast Growth Factor 21 on Energy Balance and Body Weight in a Seasonal Model of Adiposity. Journal of Neuroendocrinology 2013; 25(-2-):180-189. >>> Fibroblast growth factor-21, recomb. human; Saline; SC; Hamster; 2002; 14 days; Control animals received mp w/ vehicle; animal info (adult, male); post op. care (Rimadyl).
Q2417 Sleeman,I.J., Boshoff,E.L., Duty,S. Fibroblast growth factor-20 protects against dopamine neuron loss in vitro and provides functional protection in the 6-hydroxydopamine-lesioned rat model of Parkinson’s disease. Neuropharmacology 2012; 63(-7-):1268-1277. >>> Fibroblast growth factor-20; Serum free medium; rat serum albumin; CSF/CNS (supra-nigra); Rat; 1007D; 6 days; Control animals received mp w/ vehicle; animal info (Sprague Dawley, male, 250-300 g); cyanoacrylate used; neurodegenerative (Parkinson’s disease).
Q2411 Huang,W.H., Li,Y.D., Lin,Y.F., Ye,X., Zang,D.W. Effects of leukemia inhibitory factor and basic fibroblast growth factor on free radicals and endogenous stem cell proliferation in a mouse model of cerebral infarction. Neural Regeneration Research 2012; 7(-19-):1469-1474. >>> Leukemia inhibitory factor; fibroblast growth factor, basic; Saline, normal; Mice; 21 days; Animal info (C57BL/6, male, 8 wks old).
Q2021 Karimi-Abdolrezaee,S., Schut,D., Wang,J., Fehlings,M.G. Chondroitinase and Growth Factors Enhance Activation and Oligodendrocyte Differentiation of Endogenous Neural Precursor Cells after Spinal Cord Injury. PLoS One 2012; 7(-5-):U605-U620. >>> Epidermal growth factor; fibroblast growth factor, basic; platelet derived growth factor-AA; Saline; albumin, rat serum; CSF/CNS (intrathecal); Rat; 1007D; 7 days; Controls received mp w/ vehicle; animal info (Wistar, female, 250 g); spinal cord injury; intrathecal catheter used (0007741).
Q2020 Adams,A.C., Coskun,T., Rovira,A.R.I., Schneider,M.A., Raches,D.W., Micanovic,R., Bina,H.A., Dunbar,J.D., Kharitonenkov,A. Fundamentals of FGF19&FGF21 Action In Vitro and In Vivo. PLoS One 2012; 7(-5-):U1479-U1489. >>> Fibroblast growth factor 19; fibroblast growth factor 21; uridine, bromodeoxy; PBS; SC; Mice; 7 days; Controls received mp w/ vehicle; animal info (male, C57BL/6J, ob/ob, 9 wks old); labeling of hepatocytes.
Q1884 Jang,E., Albadawi,H., Watkins,M.T., Edelman,E.R., Baker,A.B. Syndecan-4 proteoliposomes enhance fibroblast growth factor-2 (FGF-2)-induced proliferation, migration, and neovascularization of ischemic muscle. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2012; 109(-5-):1679-1684. >>> Fibroblast growth factor-2; syndecan-4, proteoliposome; SC; Rat; 1004; 7-16 days; Controls received mp w/ PBS; animal info (Sprague Dawley); wound clips used; ischemia.
Q1741 Frontini,M.J., Nong,Z.X., Gros,R., Drangova,M., O’Neil,C., Rahman,M.N., Akawi,O., Yin,H., Ellis,C.G., Pickering,J.G. Fibroblast growth factor 9 delivery during angiogenesis produces durable, vasoresponsive microvessels wrapped by smooth muscle cells. NATURE BIOTECHNOLOGY 2011; 29(-5-):421-U232. >>> Fibroblast growth factor-9; PBS; IA (femoral); Mice; 1007D; ; Controls received mp w/ vehicle; animal info (male, C57BL/6J, 9-10 mo old); polyethylene tubing used; ischemia (hind limb).
Q1677 Wu,A.L., Kolumam,G., Stawicki,S., Chen,Y.M., Li,J., Zavala-Solorio,J., Phamluong,K., Feng,B., Li,L., Marsters,S., Kates,L., van Bruggen,N., Leabman,M., Wong,A., West,D., Stern,H., Luis,E., Kim,H.S., Yansura,D., Peterson,A.S., Filvaroff,E., Wu,Y., Sonoda,J. Amelioration of Type 2 Diabetes by Antibody-Mediated Activation of Fibroblast Growth Factor Receptor 1. Science Translational Medicine 2011; 3(-113-):U32-U41. >>> Fibroblast growth factor 21, recomb. human; PBS; SC; Mice; 2001; ; Controls received mp w/ vehicle; animal info (adult, male, female, C57BL/6J, db/db).
Q1567 Yoshikawa,G., Momiyama,T., Oya,S., Takai,K., Tanaka,J.I., Higashiyama,S., Saito,N., Kirino,T., Kawahara,N. Induction of striatal neurogenesis and generation of region-specific functional mature neurons after ischemia by growth factors Laboratory investigation. Journal of Neurosurgery 2010; 113(-4-):835-850. >>> Fibroblast growth factor-2; CSF, artificial; albumin, rat serum; CSF/CNS; Rat; 1007D; 7 days; Controls received mp w/ vehicle; animal info (Wistar, male, 8-10 wks old, 280-320 g); bilateral infusion.
Q1404 Wu,A.L., Coulter,S., Liddle,C., Wong,A.N., Eastham-Anderson,J., French,D.M., Peterson,A.S., Sonoda,J. FGF19 Regulates Cell Proliferation, Glucose and Bile Acid Metabolism via FGFR4-Dependent and Independent Pathways. PLoS One 2011; 6(-3-):U433-U443. >>> Fibroblast growth factor 19; FGF19v; SC; Mice; 2001; 7 days; Controls received mp w/ vehicle; animal info (ob/ob, C57BL/6, 11 wks old, 12-15 wks old, Fgfr wt); FGF19v is a fibroblast growth factor variant.
Q1362 Toledo,R.N., Borin,A., Cruz,O.L.M., Ho,P.L., Testa,J.R.G., Fukuda,Y. The Action of Topical Basic Fibroblast Growth Factor in Facial Nerve Regeneration. OTOLOGY&NEUROTOLOGY 2010; 31(-3-):498-505. >>> Fibroblast growth factor, basic; albumin, human; Ringer lactate solution; CSF/CNS (facial nerve anastomosis); Rat; 2002; 14 days; Controls received mp w/ sodium heparin, human albumin in Ringer lactate solution; animal info (male, Wistar, adult); functionality of mp verified by residual volume; good methods, pg 499; stress/adverse reaction: (see pg. 501) “neuroma at the anastomosis”, “hemoatoma at the surgical incision”; tissue perfusion (epineural anastomosis); vinyl catheter used; “We chose to use minipumps because this method allows better control of the quantity of bFGF delivered at the target site and is compatible with standard facial nerve surgical techniques. Furthermore, the isolate reservoir of the minipumps prevents the contact of the study drug with substances that could inactivate it” pg 502.
Q1236 Miyasaka,E.A., Raghavan,S., Gilmont,R.R., Mittal,K., Somara,S., Bitar,K.N., Teitelbaum,D.H. In vivo growth of a bioengineered internal anal sphincter: comparison of growth factors for optimization of growth and survival. PEDIATRIC SURGERY INTERNATIONAL 2011; 27(-2-):137-143. >>> Fibroblast growth factor-2; vascular endothelial growth factor-2; platelet-derived growth factor; SC; Mice; 1004; 28 days; Controls received mp with no growth factors; animal info (C57BL/6); good methods, pg 138; tissue perfusion (internal anal sphincter); silicone catheter used; “the osmotic pumps we used completed delivery of the growth factors by 28 days, which would clearly limit the duration of exposure to the growth factor, lessening the risk of malignancy” pg 143.
Q1151 Joseph,N.M., He,S.H., Quintana,E., Kim,Y.G., Nunez,G., Morrison,S.J. Enteric glia are multipotent in culture but primarily form glia in the adult rodent gut. Journal of Clinical Investigation 2011; 121(-9-):3398-3411. >>> Epidermal growth factor; fibroblast growth factor; glial-derived neurotrophic factor; BSA; PBS; IP; Rat; 7 days; Controls received mp w/ vehicle; animal info (P90).
Q0891 MacMillan,K.S., Naidoo,J., Liang,J., Melito,L., Williams,N.S., Morlock,L., Huntington,P.J., Estill,S.J., Longgood,J., Becker,G.L., McKnight,S.L., Pieper,A.A., De Brabander,J.K., Ready,J.M. Development of Proneurogenic, Neuroprotective Small Molecules. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 2011; 133(-5-):1428-1437. >>> P7C3; analogue, P7C3; fibroblast growth factor-2; DMSO; cremophor EL; dextrose; water; CSF/CNS; Mice; 1007D; 7 days; Positive controls received mp w/ FGF-2; animal info (12 week old, C57BL/J6); neurodegenerative (Alzheimer’s disease); 3% DMSO used.
Q0482 Theurl,M., Schgoer,W., Albrecht,K., Jeschke,J., Egger,M., Beer,A.G.E., Vasiljevic,D., Rong,S., Wolf,A.M., Bahlmann,F.H., Patsch,J.R., Wolf,D., Schratzberger,P., Mahata,S.K., Kirchmair,R. The Neuropeptide Catestatin Acts As a Novel Angiogenic Cytokine via a Basic Fibroblast Growth Factor-Dependent Mechanism. Circulation Research 2010; 107(-11-):1326-1U95. >>> Antibody, fibroblast growth factor, b-, mouse; SC; Mice; 3, 4 weeks; Controls received mp w/control antibody; animal info (male, C57BL/6 wild-type, 12-15 mo old).
Q0308 Raghavan,S., Miyasaka,E.A., Hashish,M., Somara,S., Gilmont,R.R., Teitelbaum,D.H., Bitar,K.N. Successful implantation of physiologically functional bioengineered mouse internal anal sphincter. American Journal of Physiology-Gastrointestinal and Liver Physiology 2010; 299(-2-):G430-G439. >>> Fibroblast growth factor-2, human, recomb; SC; Mice; 1004; 21 days; Animal info (C57BL/6J female, specific pathogen-free, 8 wk old); ALZET PE catheter used; catheter connected to physiologically functional bioengineered mouse internal anal sphincter; tissue perfusion (internal anal sphincter).
Q0106 Sarruf,D.A., Thaler,J.P., Morton,G.J., German,J., Fischer,J.D., Ogimoto,K., Schwartz,M.W. Fibroblast Growth Factor 21 Action in the Brain Increases Energy Expenditure and Insulin Sensitivity in Obese Rats. Diabetes 2010; 59(-7-):1817-1824. >>> Fibroblast growth factor-21, recomb. human; BSA; CSF/CNS; Rat; 2002; 2 weeks; Controls received mp w/ vehicle; ALZET brain infusion kit 2 used; animal info (male, Wistar, 250-275 g); Research diets D12492; diabetes.
Q0063 Hashish,M., Raghavan,S., Somara,S., Gilmont,R.R., Miyasaka,E., Bitar,K.N., Teitelbaum,D.H. Surgical implantation of a bioengineered internal anal sphincter. Journal of Pediatric Surgery 2010; 45(-1-):52-58. >>> Fibroblast growth factor-2, recomb.; PBS, sterile; SC; Mice; 1004; 25 days; Controls received mp w/ vehicle; tissue perfusion (internal anal sphincter); no stress (see pg. 57); good methods (pg. 53); animal info (female, C57BL/6, 8 wks old); “use of such pumps provided constant infusion over the postimplantation period without observed evidence of systemic effects and allowed increased efficiency of the drug owing to its proximity” pg. 57; operative photographs (pg. 54).
Q0061 Karimi-Abdolrezaee,S., Eftekharpour,E., Wang,J., Schut,D., Fehlings,M.G. Synergistic Effects of Transplanted Adult Neural Stem/Progenitor Cells, Chondroitinase, and Growth Factors Promote Functional Repair and Plasticity of the Chronically Injured Spinal Cord. Journal of Neuroscience 2010; 30(-5-):1657-1676. >>> Chondroitinase ABC; epidermal growth factor; fibroblast growth factor; platelet-derived growth factor; Penicillinase; saline; albumin, rat serum; CSF, artificial; gentamicin; BSA; CSF/CNS (intrathecal, subarachnoid space); Rat; 1007D; 7 days; Controls received mp w/ vehicle; animal info (female, Wistar, 250 g.); ALZET intrathecal catheter used (0007741).
P9843 Oya,S., Yoshikawa,G., Takai,K., Tanaka,J.I., Higashiyama,S., Saito,N., Kirino,T., Kawahara,N. ATTENUATION OF Notch SIGNALING PROMOTES THE DIFFERENTIATION OF NEURAL PROGENITORS INTO NEURONS IN THE HIPPOCAMPAL CA1 REGION AFTER ISCHEMIC INJURY. Neuroscience 2009; 158(-2-):683-692. >>> Epidermal growth factor, recomb. human; fibroblast growth factor, recomb. human; Jag1, recomb. rat; S2188; DLL4, recomb. mouse; CSF, artificial, DMSO; CSF/CNS; Rat; 1003D; 1007D; 11 days; Controls received mp w/ vehicle; pumps replaced after 3 days; enzyme inhibitor (gamma secretase); animal info (male, Wistar, adult, 8 wks old, 280-320 g.); ischemia (cerebral); bilateral infusion; 10% DMSO used; brain tissue distribution.
P9669 Sun,D, Bullock,M.R., McGinn,M.J., Zhou,Z., Altememi,N., Hagood,S., Hamm,R, Colello,R.J. Basic fibroblast growth factor-enhanced neurogenesis contributes to cognitive recovery in rats following traumatic brain injury. Experimental Neurology 2009; 216(-1-):56-65. >>> Fibroblast growth factor, b- recomb. human; CSF, artificial; CSF/CNS; Rat; 1007D; 7 days; Controls received mp w/ vehicle; ALZET brain infusion kit 2 used; animal info (male, Sprague Dawley, 3 months old, 300 g.).
P9320 De Toni,A., Zbinden,M., Epstein,J.A., Altaba,A.R.I., Prochiantz,A., Caille,I. Regulation of survival in adult hippocampal and glioblastoma stem cell lineages by the homeodomain-only protein HOP. Neural Development 2008; 3(-;-):U2-U13. >>> Epidermal growth factor; fibroblast growth factor, basic; RNA, small interfering; Saline, physiological; CSF/CNS; CSF/CNS (dentate gyrus); Rat; 1003D; 3 days; Controls received mp w/ control siRNA; cancer (glioblastoma); peptides; animal info (Swiss, HOP -/-, wt, adult); HOP or control siRNA was coupled to the cell permeant peptide, penetratin.
P9296 Zhao,H.D., Negash,L., Wei,Q., LaCour,T.G., Estill,S.J., Capota,E., Pieper,A.A., Harran,P.G. Acid promoted cinnamyl ion mobility within peptide derived macrocycles. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 2008; 130(-42-):13864-13866. >>> Fibroblast growth factor, recomb.; peptides, synthetic; cyclophane 17, meta-; CSF, artificial; CSF/CNS; Mice; 7 days; Controls received mp w/ vehicle or FGF; dose-response (fig. 3); animal info (adult, C57BL/6J); peptides.
P9180 Coskun,T., Bina,H.A., Schneider,M.A., Dunbar,J.D., Hu,C.C., Chen,Y.Y., Moller,D.E., Kharitonenkov,A. Fibroblast Growth Factor 21 Corrects Obesity in Mice. Endocrinology 2008; 149(-12-):6018-6027. >>> Fibroblast growth factor 21; SC; Mice; 2 weeks; Comparison of SC injections vs. mp; half-life (p. 6025) 1 hour in mice; peptides; animal info (male, DIO, ob/ob, C57BL/6); obesity; “approximately a 10-fold greater dose of FGF21 was required to achieve an equivalent weight reduction compared with FGF21 administration via ALZET pumps.” pg. 6021.
P8932 Oya,S., Yoshikawa,G., Takai,K., Tanaka,J.I., Higashiyama,S., Saito,N., Kirino,T., Kawahara,N. Region-specific proliferative response of neural progenitors to exogenous stimulation by growth factors following ischemia. NeuroReport 2008; 19(-8-):805-810. >>> Epidermal growth factor, recomb. human; fibroblast growth factor-2, recomb. human; insulin-like growth factor I, recomb. human; erythropoietin, recomb. rat; brain-derived neurotrophic factor, recomb. human; DDL4, recomb. mouse; CSF/CNS; Rat; 1003D; 3 days; Ischemia; animal info (male, Wistar, 8wks old, 280-300 g.); bilateral infusion.
P8930 Abbaspour,A., Takata,S., Sairyo,K., Katoh,S., Yukata,K., Yasul,N. Continuous local infusion of fibroblast growth factor-2 enhances consolidation of the bone segment lengthened by distraction osteogenesis in rabbit experiment. Bone 2008; 42(-1-):98-106. >>> Fibroblast growth factor-2, recomb. human; Bone (tibia); Rabbit; 2ML4; 14 days; half-life (p. 104) “relatively short”; tissue perfusion (tibia); animal info (male, 1.8-2.2 kg., osteotomized); polyvinyl catheter tubing used; pump image fig. 1e.
P8818 Conigliaro,A., Colletti,M., Cicchini,C., Guerra,M.T., Manfredini,R., Zini,R., Bordoni,V., Siepi,F., Leopizzi,M., Tripodi,M., Amicone,L. Isolation and characterization of a murine resident liver stem cell. CELL DEATH AND DIFFERENTIATION 2008; 15(-1-):123-133. >>> Fibroblast growth factor, bovine; Heparin; SC; Mice (nude); 10 days; Peptides.
P8702 Grumbles,R.M., Casella,G.T.B., Rudinsky,M.J., Wood,P.M., Sesodia,S., Bent,M., Thomas,C.K. Long-term delivery of FGF-6 changes the fiber type and fatigability of muscle reinnervated from embryonic neurons transplanted into adult rat peripheral nerve. Journal of Neuroscience Research 2007; 85(-9-):1933-1942. >>> Fibroblast growth factor-6, recomb. human; PBS; BSA; IM (medial gastrocnemius); Rat; 2ML4; 4 weeks; Controls received mp w/ vehicle; peptides; animal info (female, Fischer, adult).
P8661 Miller,J.M., Le Prell,C.G., Prieskorn,D.M., Wys,N.L., Altschuler,R.A. Delayed neurotrophin treatment following deafness rescues spiral ganglion cells from death and promotes regrowth of auditory nerve peripheral processes: Effects of brain-derived neurotrophic factor and fibroblast growth factor. Journal of Neuroscience Research 2007; 85(-9-):1959-1969. >>> Brain-derived neurotrophic factor; fibroblast growth factor-1; Perilymph, artificial; albumin, guinea pig serum; Ear (scala tympani); Guinea-pig; 2002; 26 days; Controls received mp w/ vehicle; pumps replaced at day 13; peptides; tissue perfusion (scala tympani); animal info (male, female, pigmented, 250-300g, deafened).
P8658 Rai,K.S., Hattiangady,B., Shetty,A.K. Enhanced production and dendritic growth of new dentate granule cells in the middle-aged hippocampus following intracerebroventricular FGF-2 infusions. European Journal of Neuroscience 2007; 26(-7-):1765-1779. >>> Fibroblast growth factor-2; CSF, artificial; CSF/CNS; Rat; 2002; 14 days; Controls received mp w/ vehicle; functionality of mp verified by residual volume; good methods (p. 1766-1767); ALZET brain infusion kit used; cyanoacrylate adhesive; post op. care (tetracycline); animal info (male, F344, 12 months).
P8631 Powell,JA Jr, Mousa,S.A. Neutrophil-Activating Protein-2- and Interieukin-8-Mediated Angiogenesis. Journal of Cellular Biochemistry 2007; 102(-2-):412-420. >>> Fibroblast growth factor-2, basic, human; penicillamine, S-nitroso N-acetyl; glutathione, S-nitroso N-acetyl; SC; Mice; 14 days; Peptides; animal info (male, C57BL/6, 6-8 wks old); SNAP and SNAG are nitric oxide donors.
P8478 Ma,T., Gutnick,J., Salazar,B., Larsen,M.D., Suenaga,E., Zilber,S., Huang,Z., Huddleston,J., Smith,R.L., Goodman,S. Modulation of allograft incorporation by growth factors over a prolonged continuous infusion of duration in vivo. Bone 2007; 41(-3-):386-392. >>> Fibroblast growth factor-2; osteogenic protein-1, recomb. human; Water, distilled; acetate buffer; Bone (tibia); Rabbit; 2004; 4,36 weeks; Controls received no treatment to contralateral side; long-term study; pumps replaced every 4 weeks, with 4 week rest periods between 4 week infusion periods; animal info (New Zealand white, 6-12 months old, 3.5-4.2 kg); peptides; OP-1 also known as bone morphogenic protein-7 (BMP-7); tissue perfusion (tibia); unique drug test chamber and bone harvest chamber used in conjunction with mp.
3. Hepatocyte Growth Factor
Q2105 Kitamura,K., Fujiyoshi,K., Yamane,J., Toyota,F., Hikishima,K., Nomura,T., Funakoshi,H., Nakamura,T., Aoki,M., Toyama,Y., Okano,H., Nakamura,M. Human Hepatocyte Growth Factor Promotes Functional Recovery in Primates after Spinal Cord Injury. PLoS One 2011; 6(-11-):U83-U95. >>> Hepatocyte growth factor, recomb. human; PBS; CSF/CNS (intrathecal); Monkey (marmoset); 2004; 4 weeks; Controls received mp w/ vehicle; animal info (adult, female, common, 295-350 g); ALZET rat intrathecal catheter used.
Q1885 Katz,M.S., Thatch,K.A., Schwartz,M.Z. Hepatocyte growth factor and omega-3-enriched feeds have a synergistic effect on mucosal mass in an animal model of inflammatory bowel disease. Journal of Pediatric Surgery 2012; 47(-1-):194-198. >>> Hepatocyte growth factor, human recomb.; PBS; HCL, tris; IV (jugular); Rat; 2002; 14 days; Controls received mp w/ saline; animal info (female, adult, HLA-B27, 200-250 g).
Q1561 Katz,M.S., Thatch,K.A., Schwartz,M.Z. Dose Variation of Hepatocyte Growth Factor and its Effects on an Animal Model of TPN-Induced Liver Injury. Journal of Surgical Research 2010; 163(-2-):294-298. >>> Hepatocyte growth factor; IV (jugular); Rat; 14 days; Controls received mp w/ saline; animal info (Sprague Dawley, female, adult); dose response.
Q0678 Thatch,K.A., Mendelson,K.G., Haber,M.M., Schwartz,M.Z. Growth Factor Manipulation of Intestinal Angiogenesis: A Possible New Paradigm in the Management of Inflammatory Bowel Disease. Journal of Surgical Research 2009; 156(-2-):245-249. >>> Hepatocyte growth factor, recomb. human; HCl, Tris; PBS; IV (jugular); Rat; 1002; 14 days; Controls received mp w/ saline; animal info (female, adult, Fisher).
P9298 Takeo,S., Takagi,N., Takagi,K., Date,I., Ishida,K., Besshoh,S., Nakamura,T., Tanonaka,K. Hepatocyte growth factor suppresses ischemic cerebral edema in rats with microsphere embolism. Neuroscience Letters 2008; 448(-1-):125-129. >>> Hepatocyte growth factor, recomb. human; Saline, physiological; CSF/CNS; Rat; 2001; 3 days; Controls received no treatment; peptides; ischemia (cerebral); animal info (male, Wistar, 220-250 g.).
P9077 Ye,L., Lewis-Russell,J.M., Sanders,A.J., Kynaston,H., Jiang,W.G. HGF/SF up-regulates the expression of bone morphogenetic protein 7 in prostate cancer cells. UROLOGIC ONCOLOGY-SEMINARS AND ORIGINAL INVESTIGATIONS 2008; 26(-2-):190-197. >>> Hepatocyte growth factor, recomb. human; NK4, recomb. human; SC; Mice (nude); 2004; 4 weeks; Cancer (prostate); animal info (athymic, male, CD-1, 4-6 wks old).
P8927 Chen,J.H., Wu,C.W., Kao,H.L., Chang,H.M., Li,A.F.Y., Liu,T.Y., Chi,C.W. Effects of COX-2 inhibitor on growth of human gastric cancer cells and its relation to hepatocyte growth factor. Cancer Letters 2006; 239(-2-):263-270. >>> Hepatocyte growth factor; IP; Mice (SCID); 28 days; Controls received sham operation; pumps replaced after 14 days; cancer (gastric); peptides; post op. care (streptomycin, penicillin); animal info (male, SCID, 6-8 wks old, 20-25 g.).
P8925 Ye,L., Lewis-Russell,J.M., Davies,G., Sanders,A.J., Kynaston,H., Jiang,W.G. Hepatocyte growth factor up-regulates the expression of the bone morphogenetic protein (BMP) receptors, BMPR-IB and BMPR-II, in human prostate cancer cells. INTERNATIONAL JOURNAL OF ONCOLOGY 2007; 30(-2-):521-529. >>> Hepatocyte growth factor, recomb. human; SC; Mice (nude); 2004; 4 weeks; Controls received mp w/ BSA buffer; cancer (prostate); peptides; animal info (female, athymic, nude, CD-1, 4-6 wks old); HGF antagonist.
P8908 Ishigaki,A., Aoki,M., Nagai,M., Warita,H., Kato,S., Kato,M., Nakamura,T., Funakoshi,H., Itoyama,Y. Intrathecal delivery of hepatocyte growth factor from amyotrophic lateral sclerosis onset suppresses disease progression in rat amyotrophic lateral sclerosis model. Journal of Neuropathology and Experimental Neurology 2007; 66(-11-):1037-1044. >>> Hepatocyte growth factor, recomb. human; PBS, sulfoxide; CSF/CNS (intrathecal, subarachnoid space); Rat (transgenic); 2002; 2004; 2, 4 weeks; Controls received mp w/ vehicle; dose-response (fig. 2); peptides; animal info (G93A Tg, 100 and 115 days old); neurodegenerative (ALS); “we examined the effects of continuous intrathecal delivery of human recombinant HGF (hrHGF) into Tg rats using implanted infusion pumps for selective and less invasive supply of HGF to the spinal cord.” (p.1038).
P8815 Jin,H.K., Yang,R.H., Zheng,Z., Romero,M., Ross,J., Bou-Reslan,H., Carano,R.A.D., Kasman,I., Mai,E., Young,J., Zha,J.P., Zhang,Z.M., Ross,S., Schwall,R., Colbern,G., Merchant,M. MetMAb, the one-armed 5D5 anti-c-Met antibody, inhibits orthotopic pancreatic tumor growth and improves survival. Cancer Research 2008; 68(-11-):4360-4368. >>> Hepatocyte growth factor, human; Dextran sulfate; IP; Mice (nude); 1002; ; Controls received mp w/ vehicle; functionality of mp verified by human HGF serum levels; long-term study; pumps replaced every 12 to 14 days; cancer (pancreatic); peptides; animal info (female, nu/nu).
P8085 Date,I., Takagi,N., Takagi,K., Tanonaka,K., Funakoshi,H., Matsumoto,K., Nakamura,T., Takeo,S. Hepatocyte growth factor attenuates cerebral ischemia-induced increase in permeability of the blood-brain barrier and decreases in expression of tight junctional proteins in cerebral vessels. Neuroscience Letters 2006; 407(-2-):141-145. >>> Hepatocyte growth factor, recomb. human; CSF/CNS; Rat; 2001; 7 days; Controls received mp w/ physiological saline; peptides; ischemia (cerebral); animal info (male, Wistar, 180-220g, microsphere-induced cerebral embolism).
P8009 Niimura,M., Takagi,N., Takagi,K., Mizutani,R., Ishihara,N., Matsumoto,K., Funakoshi,H., Nakamura,T., Takeo,S. Prevention of apoptosis-inducing factor translocation is a possible mechanism for protective effects of hepatocyte growth factor against neuronal cell death in the hippocampus after transient forebrain ischemia. Journal of Cerebral Blood Flow and Metabolism 2006; 26(-11-):1354-1365. >>> Hepatocyte growth factor; Saline; CSF/CNS (right hippocampal region); Rat; 1003D; 3 days; Animal info (male, Wistar, 200-250 grams); ischemia (cerebral); tissue perfusion (hippocampal region).
P8008 Niimura,M., Takagi,N., Takagi,K., Mizutani,R., Tanonaka,K., Funakoshi,H., Matsumoto,K., Nakamura,T., Takeo,S. The protective effect of hepatocyte growth factor against cell death in the hippocampus after transient forebrain ischemia is related to the improvement of apurinic/apyrimidinic endonuclease/redox factor-1 level and inhibition of NADPH oxidase activity. Neuroscience Letters 2006; 407(-2-):136-140. >>> Hepatocyte growth factor; CSF/CNS (right hippocampal region); Rat; 1003D; 3 days; Animal info (male, Wistar, 200-250 grams); ischemia (cerebral); tissue perfusion (hippocampal region).
P7925 Tada,T., Zhan,H., Tanaka,Y., Hongo,K., Matsumoto,K., Nakamura,T. Intraventricular administration of hepatocyte growth factor treats mouse communicating hydrocephalus induced by transforming growth factor beta 1. NEUROBIOLOGY OF DISEASE 2006; 21(-3-):576-586. >>> Hepatocyte growth factor, recomb. human; PBS; BSA; CSF/CNS; Mice; 1007D; 1002; 7,14 days; Animal info (C57BL/6, 10-days old).
P7846 Niimura,M., Takagi,N., Takagi,K., Funakoshi,H., Nakamura,T., Takeo,S. Effects of hepatocyte growth factor on phosphorylation of extracellular signal-regulated kinase and hippocampal cell death in rats with transient forebrain ischemia. European Journal of Pharmacology 2006; 535(-1-3-):114-124. >>> Hepatocyte growth factor, recomb. human; Saline, physiological; CSF/CNS (hippocampus); Rat; 1003D; 1, 2, 3 days; 0.5, 1, 6 hours; Controls received mp w/ vehicle; dose-response (fig. 2); peptides; ischemia (cerebral); animal info (male, Wistar, 200-250g., transient forebrain ischemia by carotid artery occlusion); mp primed in 37 celsius saline.
P7668 Hasuike,S., Ido,A., Uto,H., Moriuchi,A., Tahara,Y., Numata,M., Nagata,K., Hori,T., Hayashi,K., Tsubouchi,H. Hepatocyte growth factor accelerates the proliferation of hepatic oval cells and possibly promotes the differentiation in a 2-acetylaminofluorene/partial hepatectomy model in rats. Journal of Gastroenterology and Hepatology 2005; 20(-11-):1753-1761. >>> Hepatocyte growth factor, recomb. human; PBS; IP; Rat; 7 days; Controls received mp w/ vehicle; functionality of mp verified by human HGF serum levels; replacement therapy (hepatectomy); half-life (pg. 1758)<3 min; animal info (male, Fisher, 8 wk. old).
P7654 Timmapuri,S.J., Otterburn,D.M., Arafat,H., Schwartz,M.Z. Hepatocyte growth factor increases glucagon immunoreactivity in jejunal cells during intestinal adaptation. Journal of Pediatric Surgery 2006; 41(-1-):150-153. >>> Hepatocyte growth factor; IV (jugular); Rat; 2002; 14 days; Controls received mp w/ saline; animal info (male, Sprague-Dawley, adult, 200-225 g); PE catheter used.

4. Insulin-like Growth Factor
Q2675 Secco,M., Bueno,C.Jr, Vieira,N.M., Almeida,C., Pelatti,M., Zucconi,E., Bartolini,P., Vainzof,M., Miyabara,E.H., Okamoto,O.K., Zatz,M. Systemic Delivery of Human Mesenchymal Stromal Cells Combined with IGF-1 Enhances Muscle Functional Recovery in LAMA2 (dy/2j) Dystrophic Mice. Stem Cell Reviews and Reports 2013; 9(-1-):93-109. >>> Insulin-like growth factor-1, R3, long; Acetic acid; SC; Mice; 1002; 8 weeks; Control animals received mp w/ vehicle; animal info (1 mo old, B6.WK-Lama2 dy/2J); long-term study; pumps replaced every 2 weeks.
Q2623 Franco,C., Fernandez,S., Torres-Aleman,I. Frataxin deficiency unveils cell-context dependent actions of insulin-like growth factor I on neurons. Molecular Neurodegeneration 2012; 7(-;-):U1-U10. >>> Insulin-like growth factor-1; SC; Mice; 1 month; Animal info (YG8R, wt, 4-6 mo old).
Q2050 Nishizawa,H., Takahashi,M., Fukuoka,H., Iguchi,G., Kitazawa,R., Takahashi,Y. GH-independent IGF-I action is essential to prevent the development of nonalcoholic steatohepatitis in a GH-deficient rat model. Biochemical and Biophysical Research Communications 2012; 423(-2-):295-300. >>> Growth hormone; insulin-like growth factor I, recomb. human; Saline; SC; Rat; 2004; 4 weeks; Controls received mp w/ vehicle; animal info (SDR, GH-def, male, 16 wks old).
Q1988 Xian,L.L., Wu,X.W., Pang,L.J., Lou,M., Rosen,C.J., Qiu,T., Crane,J., Frassica,F., Zhang,L.M., Rodriguez,J.P., Jia,X.F., Yakar,S., Xuan,S.H., Efstratiadis,A., Wan,M., Cao,X. Matrix IGF-1 maintains bone mass by activation of mTOR in mesenchymal stem cells. Nature Medicine 2012; 18(-7-):1095-U126. >>> Insulin-like growth factor I; insulin-like growth factor binding protein; SC; Mice; 4 weeks; Controls received mp w/ vehicle; animal info (4 wks old, male, LID).
Q1521 Sun,Y., Todd,B.J., Thornton,K., Etgen,A.M., Neal-Perry,G. Differential Effects of Hypothalamic IGF-I on Gonadotropin Releasing Hormone Neuronal Activation During Steroid-Induced LH Surges in Young and Middle-Aged Female Rats. Endocrinology 2011; 152(-11-):4276-4287. >>> JB-1; insulin-like growth factor-1; CSF, artificial; CSF/CNS (third ventricle); Rat; 2002; 7 days; animal info (young , 3-4 mo old, middle-aged, retired breeders, 9-11 mo old, female Sprague Dawley, ovariohysterectomized); guide cannula used; cannula placement verified by tracking the cannula path in brain sections; artificial CSF recipe; peptides;.
Q1506 Sukhanov,S., Higashi,Y., Shai,S.Y., Blackstock,C., Galvez,S., Vaughn,C., Titterington,J., Delafontaine,P. Differential requirement for nitric oxide in IGF-1-induced anti-apoptotic, anti-oxidant and anti-atherosclerotic effects. FEBS Letters 2011; 585(-19-):3065-3072. >>> Insulin-like growth factor-1, recomb. human; Mice; 4, 8, 12 weeks; Controls received mp w/ saline; animal info (Apoe -/-, C57BL/6, 8 wks old); long-term study.
Q1502 Duarte,A.I., Petit,G.H., Ranganathan,S., Li,J.Y., Oliveira,C.R., Brundin,P., Bjoerkqvist,M., Rego,A.C. IGF-1 protects against diabetic features in an in vivo model of Huntington’s disease. Experimental Neurology 2011; 231(-2-):314-319. >>> Insulin-like growth factor-1, recomb. human; Saline; SC; Mice; 1002; 14 days; Controls received mp w/ vehicle; animal info (R6/2, wt, male, 9 wks old); neurodegenerative (Huntington’s disease).
Q1255 Perez-Martin,M., Cifuentes,M., Grondona,J.M., Lopez-Avalos,M.D., Gomez-Pinedo,U., Garcia-Verdugo,J.M., Fernandez-Llebrez,P. IGF-I stimulates neurogenesis in the hypothalamus of adult rats. European Journal of Neuroscience 2010; 31(-9-):1533-1548. >>> Insulin-like growth factor-1; thymidine, H3; CSF/CNS; Rat; 2001; 7 days; Controls received mp w/ saline; animal info (adult, male, Wistar, albino, male, female, 2 mo old); ALZET brain infusion kit used.
Q1232 Menagh,P.J., Turner,R.T., Jump,D.B., Wong,C.P., Lowry,M.B., Yakar,S., Rosen,C.J., Iwaniec,U.T. Growth Hormone Regulates the Balance Between Bone Formation and Bone Marrow Adiposity. Journal of Bone and Mineral Research 2010; 25(-4-):757-768. >>> Insulin-like growth factor 1, recomb. human; SC; Rat; 2001; 5 days; Animal info (female, Sprague-Dawley, HYPOX, 3 mo old).
Q0767 Traub,M.L., De Butte-Smith,M., Zukin,R.S., Etgen,A.M. Oestradiol and Insulin-Like Growth Factor-1 Reduce Cell Loss after Global Ischaemia in Middle-Aged Female Rats. Journal of Neuroendocrinology 2009; 21(-12-):1038-1044. >>> Insulin-like growth factor-I; Saline, sterile; CSF/CNS; Rat; 2004; 28 days; Controls received mp w/ vehicle; animal info (female, Sprague-Dawley, 9-11 mo old, adult, 300-500 g); functionality of mp verified by residual volume; ALZET brain infusion kit used; cannula placement verified by inspection of cryostat sectioning; neuroprotection.
Q0683 Demonbreun,A.R., Fahrenbach,J.P., Deveaux,K., Earley,J.U., Pytel,P., McNally,E.M. Impaired muscle growth and response to insulin-like growth factor 1 in dysferlin-mediated muscular dystrophy. Human Molecular Genetics 2011; 20(-4-):779-789. >>> Insulin-like growth factor-I; 28 days; Controls received mp w/ PBS; animal info (Dysferlin null, wt).
Q0576 Serbedzija,P., Madl,J.E., Ishii,D.N. Insulin and IGF-I prevent brain atrophy and DNA loss in diabetes. Brain Research 2009; 1303(-;-):179-194. >>> Insulin; insulin-like growth factor-I; CSF/CNS; Rat; 12 weeks; Controls received mp w/ aCSF; animal info (Wistar, 275-300 g adult, 9 wks old, male, STZ-induced diabetes); long-term study; pumps replaced at two week intervals; ALZET brain infusion kit used; post op. care (Buprenorphine); cannula placement verified by injecting 20 ul dye through the catheter at time of assay.
Q0466 Cittadini,A., Monti,M.G., Castiello,M.C., D’Arco,E., Galasso,G., Sorriento,D., Saldamarco,L., De Paulis,A., Napoli,R., Iaccarino,G., Sacca,L. Insulin-like growth factor-1 protects from vascular stenosis and accelerates re-endothelialization in a rat model of carotid artery injury. JOURNAL OF THROMBOSIS AND HAEMOSTASIS 2009; 7(-11-):1920-1928. >>> Insulin-like growth factor-1; SC; Rat; ; Controls received mp w/saline; animal info (Sprague-Dawley, male, 210-250 g, ballon injury); 2.0 ul/h rate of infusion.
Q0345 Marino,G., Ugalde,A.P., Fernandez,A.F., Osorio,F.G., Fueyo,A., Freije,J.M.P., Lopez-Otin,C. Insulin-like growth factor 1 treatment extends longevity in a mouse model of human premature aging by restoring somatotroph axis function. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2010; 107(-37-):16268-16273. >>> Insulin-like growth factor-1, human, recomb.; HCl; saline, isotonic, sterile; SC; Mice; 1004; 56 days; Animal info (transgenic, Zmpste24 metalloproteinase deficient); long-term study; pumps replaced after 28 days.
Q0154 Apel,P.J., Ma,J.J., Callahan,M., Northam,C.N., Alton,T.B., Sonntag,W.E., Li,Z.Y. EFFECT OF LOCALLY DELIVERED IGF-1 ON NERVE REGENERATION DURING AGING: AN EXPERIMENTAL STUDY IN RATS. Muscle&Nerve 2010; 41(-3-):335-341. >>> Insulin-like growth factor-I, recomb. human; CSF/CNS (tibial nerve); Rat; 2004; 12 weeks; Controls received mp w/ saline; long-term study; pumps replaced after 6 weeks; animal info (Fischer 344 x Brown Norway, Sprague Dawley; 8 months old, 24 months old); diagram of pump with custom-made T-tube.
Q0153 Moreau,M.L., Sauvant,J., Moos,F., Palin,K. Brain insulin growth factor-I induces diuresis increase through the inhibition of arginin-vasopressin release in aged rats. NEUROBIOLOGY OF AGING 2010; 31(-3-):532-536. >>> Insulin-like growth factor-1, recomb. rat; CSF, artificial; CSF/CNS (third ventricle); Rat; 2ML4; 28 days; Animal info (male, Wistar, 3 months old, 645 g.); guide cannula used; aCSF recipe.
Q0120 Hewitt,S.C., Li,Y., Li,L.P., Korach,K.S. Estrogen-mediated Regulation of Igf1 Transcription and Uterine Growth Involves Direct Binding of Estrogen Receptor-alpha to Estrogen-responsive Elements. Journal of Biological Chemistry 2010; 285(-4-):2676-2685. >>> Insulin-like growth factor-1, synthetic long R3; Acetic acid; saline; IP; Mice; 18, 24 hours; Animal info (female, ERaKO KIKO, wt, 10 weeks old); peptides; replacement therapy (ovariectomy).
Q0078 Todd,B.J., Merhi,Z.O., Shu,J., Etgen,A.M., Neal-Perry,G.S. Hypothalamic Insulin-Like Growth Factor-I Receptors Are Necessary for Hormone-Dependent Luteinizing Hormone Surges: Implications for Female Reproductive Aging. Endocrinology 2010; 151(-3-):1356-1366. >>> JB-1; insulin-like growth factor-1; CSF, artificial; CSF/CNS (third ventricle); Rat; 2002; ; Controls received mp w/ vehicle; animal info (young, 3-4 months old, middle aged, 9-11 months old, adult, female, Sprague Dawley, ovariectomy); Plastics One cannula used; cannula placement verified post mortem by injecting dye into the cannula.
Q0057 Selvamani,A., Sohrabji,F. The Neurotoxic Effects of Estrogen on Ischemic Stroke in Older Female Rats Is Associated with Age-Dependent Loss of Insulin-Like Growth Factor-1. Journal of Neuroscience 2010; 30(-20-):6852-6861. >>> Insulin-like growth factor-1; JB-1; CSF, artificial; CSF/CNS; Rat (pregnant); 1007D; 7 days; Controls received mp w/ vehicle; ALZET brain infusion kit 2 used; cyanoacrylate adhesive; animal info (virgin, 3-4 months old, pregnant, 5-6 months old, retired, 9-11 months old); MCAO.
P9927 Cleveland,B.M., Weber,G.M., Blemings,K.P., Silverstein,J.T. Insulin-like growth factor-I and genetic effects on indexes of protein degradation in response to feed deprivation in rainbow trout (Oncorhynchus mykiss). American Journal of Physiology-Regulatory Integrative and Comparative Physiology 2009; 297(-5-):R1332-R1342. >>> Insulin-like growth factor-1, recomb. human; IP; Fish; 1003D; ; Post op.care (triple antibiotic ointment); animal info (rainbow trout.1 year old); wound clips used.
P9922 Van Mieghem,T., van Bree,R., Van Herck,E., Deprest,J., Verhaeghe,J. Insulin-like growth factor-II regulates maternal hemodynamic adaptation to pregnancy in rats. American Journal of Physiology-Regulatory Integrative and Comparative Physiology 2009; 297(-5-):R1615-R1621. >>> Insulin-like growth factor II, human, recomb.; Saline; SC; Rat (pregnant); 2001; 7 days; Animal info (4 wks old, female, Sprague Dawley).
P9784 Gehrig,S.M., Ryall,J.G., Schertzer,J.D., Lynch,G.S. Insulin-like growth factor-I analogue protects muscles of dystrophic mdx mice from contraction-mediated damage. Experimental Physiology 2008; 93(-11-):1190-1198. >>> Insulin-like growth factor I, LR-; Saline; HCl; SC; Mice; 1004; 4 weeks; Controls received mp w/ vehicle; functionality of mp verified by residual volume; animal info (male, BL/10, mdx, dystrophic, 8-10 wks old).
P9701 Duman,C.H., Schlesinger,L., Terwilliger,R., Russell,D.S., Newton,S.S., Duman,R.S. Peripheral insulin-like growth factor-I produces antidepressant-like behavior and contributes to the effect of exercise. Behavioural Brain Research 2009; 198(-2-):366-371. >>> Insulin-like growth factor I, recomb. human; antibody, anti-IGF-I, polyclonal; Saline; SC; Mice; 1002; 2004; 14, 28 days; Controls received mp w/vehicle; animal info (male, C57BL/6, 10 wks old); behavioral testing (running wheel test, forced swim test).
P9605 Fan,Y., Menon,R.K., Cohen,P., Hwang,D., Clemens,T., DiGirolamo,D.J., Kopchick,J.J., Le Roith,D., Trucco,M., Sperling,M.A. Liver-specific Deletion of the Growth Hormone Receptor Reveals Essential Role of Growth Hormone Signaling in Hepatic Lipid Metabolism. Journal of Biological Chemistry 2009; 284(-30-):19937-19944. >>> Insulin-like growth factor I, recomb. human; SC; Mice; 2 weeks; Controls received mp/placebo; animal info (16 wks old, male, GHRLD).
P9506 Fowlkes,JL. Effects of systemic and local administration of recombinant human IGF-I (rhIGF-I) on de novo bone formation in an aged mouse model. Journal of Bone and Mineral Research 2006; 21(-9-):1359-1366. >>> Insulin-like growth factor I, recomb. human; dye, methylene blue; PBS; SC; bone (tibia); bone (fibula); Mice; 1002; 14 days; Controls received mp w/ vehicle; functionality of mp verified by rhIGF-I serum levels; replacement therapy (mid-diaphyseal osteotomy (tibia, fibula)); peptides; post op. care (buprenex); tissue perfusion (tibia, fibula); animal info (male, C57BL/6, 18 months old).
P9251 Rosendahl,A.H., Holly,J.M.P., Celander,M., Forsberg,G. Systemic IGF-I administration stimulates the in vivo growth of early, but not advanced, renal cell carcinoma. International Journal of Cancer 2008; 123(-6-):1286-1291. >>> Insulin-like growth factor I, recomb. human; Saline, sterile; albumin, mouse serum; SC; Mice (SCID); 1007D; 14 days; Controls received mp w/ vehicle; cancer (renal carcinoma); peptides; animal info (female, SCID CoB-17, 8-12 wks old, 20 g.).
P9141 Seigel,G.M., Lupien,S.B., Campbell,L.A., Ishii,D.N. Systemic IGF-I treatment inhibits cell death in diabetic rat retina. Journal of Diabetes and Its Complications 2006; 20(-3-):196-204. >>> Insulin-like growth factor I; streptozotocin; Acetate; SC; Rat; 2002; 8 weeks; Controls received mp w/ vehicle; long-term study; animal info (Wistar, 12 wks old); diabetes.
P9112 Chu,Y.W., Schmitz,S., Choudhury,B., Telford,W., Kapoor,V., Garfield,S., Howe,D., Gress,R.E. Exogenous insulin-like growth factor 1 enhances thymopoiesis predominantly through thymic epithelial cell expansion. Blood 2008; 112(-7-):2836-2846. >>> Insulin-like growth factor I; Sucrose; serum, mouse, PBS; SC; Mice; 4 weeks, 14 days; Pumps replaced after 2 weeks; peptides; animal info (C57BL/6, 8-12 wks old, thymectomized, PSGL-1KO).
P9063 Iikubo,M., Kobayashi,A., Kojima,I., Ikeda,H., Sakamoto,M., Sasano,T. Excessive lateral dental arch expansion in experimentally developed acromegaly-like rats. Archives of Oral Biology 2008; 53(-10-):924-927. >>> Insulin-like growth factor I, recomb. human; Saline; SC; Rat; 2002; 4 weeks; Controls received mp w/ vehicle; peptides; animal info (10 wks old, male, Wistar, 200-236 g.).
P8968 Zeinoaldin,S., Swarts,J.J.M., Van de Heijning,B.J.M. Central application of IGF-1 postpones time of vaginal opening in normally fed, but not in food-restricted rats. Hormone Research 2006; 66(-4-):169-174. >>> Insulin-like growth factor I, recomb. human; Acetic acid; BSA; CSF/CNS; Rat; 2002; 14 days; Controls received mp w/ saline; peptides; animal info (Wistar, female, 23 days old, 45 g.); cannula position confirmed with Evans blue dye post mortem; endocrinology.
P8932 Oya,S., Yoshikawa,G., Takai,K., Tanaka,J.I., Higashiyama,S., Saito,N., Kirino,T., Kawahara,N. Region-specific proliferative response of neural progenitors to exogenous stimulation by growth factors following ischemia. NeuroReport 2008; 19(-8-):805-810. >>> Epidermal growth factor, recomb. human; fibroblast growth factor-2, recomb. human; insulin-like growth factor I, recomb. human; erythropoietin, recomb. rat; brain-derived neurotrophic factor, recomb. human; DDL4, recomb. mouse; CSF/CNS; Rat; 1003D; 3 days; Ischemia; animal info (male, Wistar, 8wks old, 280-300 g.); bilateral infusion.
P8922 Escartin,C., Boyer,F., Bemelmans,A.P., Hantraye,P., Brouillet,E. IGF-1 exacerbates the neurotoxicity of the mitochondrial inhibitor 3NP in rats. Neuroscience Letters 2007; 425(-3-):167-172. >>> Insulin-like growth factor I; nitropropionic acid, 3-; Acetic acid; BSA; SC; Rat; 2001; 2ML1; 5 days; Controls received mp w/ vehicle; functionality of mp verified by plasma glucose; dose-response (fig. 1, pg. 169); ALZET brain infusion kit used; animal info (Lewis, 400 g.); neurodegenerative (Huntington’s Disease).
P8906 Todd,B.J., Fraley,G.S., Peck,A.C., Schwartz,G.J., Etgen,A.M. Central insulin-like growth factor 1 receptors play distinct roles in the control of reproduction, food intake, and body weight in female rats. Biology of Reproduction 2007; 77(-3-):492-503. >>> JB-1; insulin-like growth factor I, human; CSF, artificial; CSF/CNS; Rat; 1007D; 2002; 5, 7, 14 days; Controls received mp w/ vehicle; stress/adverse reaction: (see pg. 494) surgery led to a 10-15% loss of presurgical body weight, temporary disruption of estrous cycles; peptides; animal info (female, Sprague Dawley, 170-190 g.); IGF-1 receptor antagonist; cannula placement verified by dye injection at end of experiment; endocrinology.
P8871 Sferruzzi-Perri,A.N., Owens,J.A., Standen,P., Roberts,C.T. Maternal insulin-like growth factor-II promotes placental functional development via the type 2 IGF receptor in the guinea pig. PLACENTA 2008; 29(-4-):347-355. >>> Insulin-like growth factor II; Acetic acid; SC; Guinea-pig; 2002; 18 days; Controls received mp w/ vehicle; animal info (pregnant, female, 500g., 3-4 months old).
P8836 Nichols,T.C., Busby,WH Jr, Merricks,E., Sipos,J., Rowland,M., Sitko,K., Clemmons,D.R. Protease-resistant insulin-like growth factor (IGF)-Binding protein-4 inhibits IGF-I actions and neointimal expansion in a porcine model of neointimal hyperplasia. Endocrinology 2007; 148(-10-):5002-5010. >>> Insulin-like growth factor-binding protein-4; Insulin-like growth factor-binding protein-4, mutated; Insulin-like growth factor-1, recomb. human; PBS; IA (carotid); IA (femoral); Pig; 21 days; Controls received mp w/ vehicle; cardiovascular; peptides; animal info (male, female, spotted Poland/China, 12 months old); protease resistant mutant form of IGFBP-4.
P8827 Sinkevicius,K.W., Burdette,J.E., Woloszyn,K., Hewitt,S.C., Hamilton,K., Sugg,S.L., Temple,K.A., Wondisford,F.E., Korach,K.S., Woodruff,T.K., Greene,G.L. An estrogen receptor-alpha; knock-in mutation provides evidence of ligand-independent signaling and allows modulation of ligand-induced pathways in vivo. Endocrinology 2008; 149(-6-):2970-2979. >>> Insulin-like growth factor I, R3-; Acetic acid; IP; Mice; 1003D; 16-24 hours; Controls received IP vehicle or E2 injections; replacement therapy (ovariectomy); peptides; animal info (female, C57/BL6 wt, ENERKI, 12 wks old); endocrinology.
P8682 Sferruzzi-Perri,A.N., Owens,J.A., Standen,P., Taylor,R.L., Robinson,J.S., Roberts,C.T. Early pregnancy maternal endocrine insulin-like growth factor I programs the placenta for increased functional capacity throughout gestation. Endocrinology 2007; 148(-9-):4362-4370. >>> Insulin-like growth factor I; insulin-like growth factor II, recomb. human; Acetic acid; SC; Guinea-pig; 2002; 18 days; Controls received mp w/ vehicle; functionality of mp verified by plasma levels; animal info (pregnant, female, 500g, 3-4 weeks); endocrinology.
P8617 Carro,E., Trejo,J.L., Busiguina,S., Torres-Aleman,I. Circulating Insulin-Like Growth Factor I Mediates the Protective Effects of Physical Exercise against Brain Insults of Different Etiology and Anatomy. Journal of Neuroscience 2001; 21(-15-):5678-5684. >>> Antibody, anti-IGF-I; serum, rabbit; insulin-like growth factor I, recomb.; Saline; SC; Rat; mice; 1002; 2004; 4-6 weeks; 14 days; Controls received mp w/ normal rabbit serum; long-term study; peptides; animal info (male, C57BL/6, 25 grams, male, wistar, 250-300 grams).
P8614 Trejo,J.L., Carro,E., Torres-Aleman,I. Circulating Insulin-Like Growth Factor I Mediates Exercise-Induced Increases in the Number of New Neurons in the Adult Hippocampus. Journal of Neuroscience 2001; 21(-5-):1628-1634. >>> Insulin-like growth factor I; antiserum, anti-IGF-I; serum, normal rabbit; Saline; SC; Rat; 1002; 2001; 7,14 days; Controls received mp w/ vehicle or normal rabbit serum; peptides neurodegenerative (Alzheimer’s disease); animal info (male, Wistar, 300 grams).
P8592 Scheiwiller,E., Guler,H.P., Merryweather,J., Scandella,C., Maerki,W., Zapf,J., Froesch,E.R. Growth restoration of insulin-deficient diabetic rats by recombinant human insulin-like growth factor I. Nature 1986; 323(-6084-):169-171. >>> Insulin-like growth factor I, recomb. human; insulin; growth hormone, human; Saline; acetic acid; Rat; 2001; 6 days; Replacement therapy (STZ-induced diabetes); dose-response (fig. 1); peptides; animal info (male, Tif RAI, 120-130 grams); endocrinology.
P8387 Serose,A., Salmon,A., Fiszman,M.Y., Fromes,Y. Short-term treatment using insulin-like growth factor-1 (IGF-1) improves life expectancy of the delta-sarcoglycan deficient hamster. JOURNAL OF GENE MEDICINE 2006; 8(-8-):1048-1055. >>> Insulin-like growth factor I, recomb. human; SC; Hamster; 2002; 19 days; Controls received mp w/ Saline; cardiovascular; peptides; animal info (male, Syrian wt or CHF147, 7-8 months old, 100-150g.).
P8385 Sferruzzi-Perri,A.N., Owens,J.A., Standen,P., Taylor,R.L., Heinemann,G.K., Robinson,J.S., Roberts,C.T. Early treatment of the pregnant guinea pig with IGFs promotes placental transport and nutrient partitioning near term. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM 2007; 292(-3-):E668-E676. >>> Insulin-like growth factor I; Insulin-like growth factor II, recomb. human; Acetic acid; SC; Guinea-Pig (pregnant); 2002; 18 days; Controls received mp w/ vehicle; functionality of mp verified by IGF-I/II maternal circulation levels; peptides; animal info (female, pregnant, 500g., 3-4 months old, gestation days 20-38).
P8370 Lopez-Lopez,C., Dietrich,M.O., Metzger,F., Loetscher,H., Torres-Aleman,I. Disturbed cross talk between insulin-like growth factor I and AMP-activated protein kinase as a possible cause of vascular dysfunction in the amyloid precursor protein/presenilin 2 mouse model of Alzheimer’s disease. Journal of Neuroscience 2007; 27(-4-):824-831. >>> Insulin-like growth factor I, recomb. human; SC; Mice; 1002; ; Controls received mp w/ saline; functionality of mp verified by serum IGF-I levels; peptides; animal info (C57BL/6J, APP/PS2, 6 month old); neurodegenerative (Alzheimer’s disease).
P8339 Schertzer,J.D., Lynch,G.S. Comparative evaluation of IGF-I gene transfer and IGF-I protein administration for enhancing skeletal muscle regeneration after injury. Gene Therapy 2006; 13(-23-):1657-1664. >>> Insulin-like growth factor I; SC; Mice; 1002; 3, 10, 14 days; Controls received non-coding plasmid; functionality of mp verified by residual volume; comparison of gene transfer vs. mp; peptides; animal info (male, C57BL10, 12-14 weeks old, myotoxic tibialis anterior muscle injury).
P8170 Kobayashi,A., Iikubo,M., Kojima,I., Ikeda,H., Sakamoto,M., Sasano,T. Morphological and histopathological changes in tongues of experimentally developed acromegaly-like rats. Hormone and Metabolic Research 2006; 38(-3-):146-151. >>> Insulin-like growth factor I, recomb. human; Saline; SC; Rat; 2002; 4 weeks; Controls received mp w/ vehicle; peptides; animal info (male, Wistar, 10 weeks old, 225 grams).
P8049 Kleinberg,D.L., Ruan,W.F., Yee,D., Kovacs,K.T., Vidal,S. Insulin-like growth factor (IGF)-I controls prostate fibromuscular development: IGF-I inhibition prevents both fibromuscular and glandular development in eugonadal mice. Endocrinology 2007; 148(-3-):1080-1088. >>> Insulin-like growth factor I; growth hormone, bovine; insulin-like growth factor-1, binding protein; SC; Mice; 1007D; 7 days; Controls received mp w/ saline; peptides; animal info (ORX, 10 weeks old, male); drugs delivered alone or in combination.

5. Nerve Growth Factor
Q2571 Mousa,S.A., Shaqura,M., Khalefa,B.I., Zoellner,C., Schaad,L., Schneider,J., Shippenberg,T.S., Richter,J.F., Hellweg,R., Shakibaei,M., Schaefer,M. Rab7 Silencing Prevents mu-Opioid Receptor Lysosomal Targeting and Rescues Opioid Responsiveness to Strengthen Diabetic Neuropathic Pain Therapy. Diabetes 2013; 62(-4-):1308-1319. >>> Nerve growth factor, beta; CSF, artificial; albumin, rat serum; CSF/CNS (intrathecal); Rat; 2001; 7 days; Control animals received mp w/ vehicle; animal info (Wistar, male, STZ induced diabetes).
Q2225 Aboulkassim,T., Tong,X.K., Tse,Y.C., Wong,T.P., Woo,S.B., Neet,K.E., Brahimi,F., Hamel,E., Saragovi,H.U. Ligand-Dependent TrkA Activity in Brain Differentially Affects Spatial Learning and Long-Term Memory. MOLECULAR PHARMACOLOGY 2011; 80(-3-):498-508. >>> Nerve growth factor, recomb; nerve growth factor TrkA agonist; CSF, artificial; CSF/CNS; Mice; 1002; 2 weeks; Controls received mp w/ vehicle; animal info (4-5 mo old, 670/671 KM-NL, 717 V-F); post op. care (buprenorphine); aCSF recipe; behavioral testing (Morris water maze test).
Q2205 Liu,J.H., Li,J.D., Lu,J., Xing,J.H., Li,J.H. Contribution of nerve growth factor to upregulation of P2X(3) expression in DRG neurons of rats with femoral artery occlusion. American Journal of Physiology-Heart and Circulatory Physiology 2011; 301(-3-):H1070-H1079. >>> Nerve growth factor; Saline; IM (hindlimb); Rat; 1003D; 3 days; Controls received mp w/ vehicle to opposite leg; animal info (Sprague Dawley, male, 5-7 wks old); “Note that the pumps were placed in the femoral triangle region and outlet of the pump was 2-3 mm distal to the inguinal ligament” pg H1071.
Q1986 Cabeza,C., Figueroa,A., Lazo,O.M., Galleguillos,C., Pissani,C., Klein,A., Gonzalez-Billault,C., Inestrosa,N.C., Alvarez,A.R., Zanlungo,S., Bronfman,F.C. Cholinergic Abnormalities, Endosomal Alterations and Up-Regulation of Nerve Growth Factor Signaling in Niemann-Pick Type C Disease. Molecular Neurodegeneration 2012; 7(-;-):U1-U18. >>> Nerve growth factor; CSF, artificial; CSF/CNS; Mice; 1002; 7 days; Controls received mp w/ vehicle; animal info (BALB/c, NPC/1 -/-); aCSF recipe; brain infusion kit used.
Q1126 Hu,Z.Q., Ulfendahl,M., Prieskorn,D.M., Olivius,P., Miller,J.M. Functional Evaluation of a Cell Replacement Therapy in the Inner Ear. OTOLOGY&NEUROTOLOGY 2009; 30(-4-):551-558. >>> Nerve growth factor; Hank’s based salt solution; albumin, guinea pig serum; Ear (cochlea); Guinea pig; 2002; ; Controls received mp w/ vehicle; animal info (pigmented, adult, 270-470 g); pumps replaced afer 13 days; post op. care (daily injections of cyclosporin and doxycycline); tissue perfusion.
Q0685 Toda,S., Sakai,A., Ikeda,Y., Sakamoto,A., Suzuki,H. A local anesthetic, ropivacaine, suppresses activated microglia via a nerve growth factor-dependent mechanism and astrocytes via a nerve growth factor-independent mechanism in neuropathic pain. Molecular Pain 2011; 7(-;-):U1-U11. >>> Nerve growth factor, beta, recomb., rat; Saline; albumin, rat, serum; CSF/CNS (intrathecal); Rat; 2001; 7 days; Controls received mp w/ vehicle; animal info (male, Sprague-Dawley, 220-300 g); chronic constrictive injury (CCI).
Q0682 Cirillo,G., Bianco,M.R., Colangelo,A.M., Cavaliere,C., Daniele,D., Zaccaro,L., Alberghina,L., Papa,M. Reactive astrocytosis-induced perturbation of synaptic homeostasis is restored by nerve growth factor. NEUROBIOLOGY OF DISEASE 2011; 41(-3-):630-639. >>> Nerve growth factor, beta, recomb.; GM6001; CSF, artificial; albumin, rat serum; CSF/CNS (intrathecal, subarachnoid space); Rat; 2001; 7 days; Controls received mp w/ vehicle; animal info (Sprague-Dawley, male, 250-300 g); enzyme inhibitor (metalloproteinase); PE10 connected to PE60 tubing.
Q0668 Beguin,P.C., El-Helou,V., Gillis,M.A., Duquette,N., Gosselin,H., Brugada,R., Villeneuve,L., Lauzier,D., Tanguay,J.F., Ribuot,C., Calderone,A. Nestin((+)) Stem Cells Independently Contribute to Neural Remodelling of the Ischemic Heart. Journal of Cellular Physiology 2011; 226(-5-):1157-1165. >>> Nerve growth factor, 2.5S; SC; Rat; 2ML1; 1 week; Controls received mp w/ saline; animal info (adult, male, Sprague-Dawley).
Q0544 Hobara,N., Yoshida,N., Goda,M., Yokomizo,A., Kitamura,Y., Sendou,T., Kawasaki,H. Neurotrophic Effect of Hepatic Growth Factor (HGF) on Reinnervation of Perivascular Calcitonin Gene-Related Peptide (CGRP)-Containing Nerves Following Phenol-Induced Nerve Injury in the Rat Mesenteric Artery. JOURNAL OF PHARMACOLOGICAL SCIENCES 2008; 108(-4-):495-504. >>> Hepatic growth factor, human; nerve growth factor; PBS; Triton-X; saline, sterile; IP; Rat; 2001; 7 days; Controls received mp w/ saline; animal info (8 wks old, Wistar).
Q0468 Conner,J.M., Franks,K.M., Titterness,A.K., Russell,K., Merrill,D.A., Christie,B.R., Sejnowski,T.J., Tuszynski,M.H. NGF Is Essential for Hippocampal Plasticity and Learning. Journal of Neuroscience 2009; 29(-35-):10883-10889. >>> Nerve growth factor, recomb. human; CSF, artificial; albumin, rat; CSF/CNS (septum); Rat; 2002; ; Controls received mp w/ vehicle; animal info (adult, female F344, 175-200 g, 3-4 months old); behavioral testing (Morris water maze test).
Q0157 Lazo,O.M., Mauna,J.C., Pissani,C.A., Inestrosa,N.C., Bronfman,F.C. Axotomy-induced neurotrophic withdrawal causes the loss of phenotypic differentiation and downregulation of NGF signalling, but not death of septal cholinergic neurons. Molecular Neurodegeneration 2010; 5(-;-):U1-U12. >>> Nerve growth factor; CSF, artificial; CSF/CNS; Rat; 2ML2; 14 days; Controls received mp w/ vehicle; animal info (Sprague Dawley, 280-300 g.); aCSF recipe; incorrectly stated 2002 pump.
Q0129 Goda,M., Atagi,S., Amitani,K., Hobara,N., Kitamura,Y., Kawasaki,H. Nerve Growth Factor Suppresses Prostate Tumor Growth. JOURNAL OF PHARMACOLOGICAL SCIENCES 2010; 112(-4-):463-466. >>> Nerve growth factor; SC; Mice (nude); 1002; 2 weeks; Controls received mp w/ saline; cancer (prostate); animal info (BALB/c, Slc, 5 wks old, nu/nu).
Q0009 Cirillo,G., Cavaliere,C., Bianco,M.R., De Simone,A., Colangelo,A.M., Sellitti,S., Alberghina,L., Papa,M. Intrathecal NGF Administration Reduces Reactive Astrocytosis and Changes Neurotrophin Receptors Expression Pattern in a Rat Model of Neuropathic Pain. Cellular and Molecular Neurobiology 2010; 30(-1-):51-62. >>> Nerve growth factor, b-, recomb. rat; CSF, artificial; CSF/CNS (intrathecal); Rat; 2001; 7 days; Controls received mp w/ vehicle; peptides; animal info (adult, male, Sprague-Dawley, 250-300 g); SCI; spinal cord injury; behavioral testing (thermal nociceptive testing, mechanical allodynia).
P9872 Unezaki,S., Yoshii,S., Mabuchi,T., Saito,A., Ito,S. Effects of neurotrophic factors on nerve regeneration monitored by in vivo imaging in thy1-YFP transgenic mice. Journal of Neuroscience Methods 2009; 178(-2-):308-315. >>> Nerve growth factor; glial-derived neurotrophic factor; Saline; CSF/CNS (sciatic nerve); Mice (transgenic); 1004; 4 weeks; Controls received mp w/ vehicle; half-life (p. 308) “short”; animal info (10 wks old, 20 g., Thy1-YEP); image of pump pg. 309; schematic of drug delivery system, fig. 1); “Because of the short biological half-life of neurotrophic factors, a delivery system that protects the protein and slowly releases it locally over a prolonged period of time is required.” pg. 308; tissue perfusion (sciatic nerve).
P9539 Xanthos,D.N., Kumar,N., Theodorsson,E., Coderre,T.J. The roles of nerve growth factor and cholecystokinin in the enhancement of morphine analgesia in a rodent model of central nervous system inflammation. Neuropharmacology 2009; 56(-3-):684-691. >>> Nerve growth factor, beta; CSF, artificial; CSF/CNS (intrathecal); Rat; 2001; 7 days; Controls received mp w/ vehicle; animal info (male, Long Evans, hooded).
P9516 Wissman,A.M., Brenowitz,E.A. The Role of Neurotrophins in the Seasonal-Like Growth of the Avian Song Control System. Journal of Neuroscience 2009; 29(-20-):6461-6471. >>> TrkB-Fc; TrkC-Fc; brain-derived neurotrophic factor; nerve growth factor; PBS; CSF/CNS (robust nucleus of the arcopallium); Bird; 1002; ; Controls received mp w/ vehicle; functionality of mp verified by residual volume; ALZET brain infusion kit 2 used; animal info (sparrow, adult, male, Gambel’s white crowned).
P9466 Xing,J.H., Lu,J., Li,J.H. Contribution of nerve growth factor to augmented TRPV1 responses of muscle sensory neurons by femoral artery occlusion. American Journal of Physiology-Heart and Circulatory Physiology 2009; 296(-5-):H1380-H1387. >>> Nerve growth factor; SC; Rat; 1003D; 72 hours; Controls received mp w/ saline to contralateral leg; cardiovascular; peptides; multiple pumps per animal (2); ischemia (muscle); animal info (male, Sprague Dawley, 5-7 wks old); SC in the hindlimb.
P9324 Averill,S., Inglis,J.J., King,V.R., Thompson,S.W.N., Cafferty,W.B.J., Shortland,P.J., Hunt,S.P., Kidd,B.L., Priestley,J.V. Reg-2 expression in dorsal root ganglion neurons after adjuvant-induced monoarthritis. Neuroscience 2008; 155(-4-):1227-1236. >>> Nerve growth factor, recomb. human; glial-derived neurotrophic factor, recomb. human; leukemia inhibitory factor, recomb. human; Saline; albumin, rat serum; CSF/CNS (intrathecal); Rat; 2002; 14 days; Controls received mp w/ vehicle; peptides, animal info (male, Wistar, 220-400 g.).
P9004 Clark,R.S.B., Nathaniel,P.D., Zhang,X.P., Dixon,C.E., Alber,S.M., Watkins,S.C., Melick,J.A., Kochanek,P.M., Graham,S.H. boc-Aspartyl(OMe)-fluoromethylketone attenuates mitochondrial release of cytochrome c and delays brain tissue loss after traumatic brain injury in rats. Journal of Cerebral Blood Flow and Metabolism 2007; 27(-2-):316-326. >>> Nerve growth factor; CSF, artificial; albumin, mouse; CSF/CNS (parietal cortex); Rat; 2002; 14 days; Controls received mp w/ vehicle; no stress (see pg. 321); peptides; cardiovascular; animal info (male, Sprague Dawley, 280-400 g., CCI brain injury); behavioral testing (beam balance, beam walking, Morris water maze).
P8890 Hobara,N., Goda,M., Yoshida,N., Takatori,S., Kitamura,Y., Mio,M., Kawasaki,H. Angiotensin II type 2 receptors facilitate reinnervation of phenol-lesioned vascular calcitonin gene-related peptide-containing nerves in rat mesenteric arteries. Neuroscience 2007; 150(-3-):730-741. >>> Angiotensin II; nerve growth factor; PD123319; losartan; Saline; water; IP; Rat; 2001; 7 days; Controls received mp w/ vehicle; functionality of mp verified by systolic blood pressure; peptides; post op. care (penicillin); animal info (8 wks old, Wistar).
P8576 Villoslada,P., Hauser,S.L., Bartke,I., Unger,J., Heald,N., Rosenberg,D., Cheung,S.W., Mobley,W.C., Fisher,S., Genain,C.P. Human Nerve Growth Factor Protects Common Marmosets against Autoimmune Encephalomyelitis by Switching the Balance of T Helper Cell Type 1 and 2 Cytokines within the Central Nervous System. Journal of Experimental Medicine 2000; 191(-10-):1799-1806. >>> Saline; nerve growth factor, recomb. human; cytochrome C; CSF/CNS; Marmoset; 2004; 35-42 days; Controls received mp w/ cytochrome C; functionality of mp verified by CSF levels of rhNGF; pumps replaced after 7-14 days of saline; immunology; ALZET brain infusion kit used; peptides; animal info (callithrix jacchus); “we chose to use an intracranial route to ensure accurate delivery of the drug into the CNS. This resulted in sustained elevated concentrations of rhNGF in the CSF of all rhNGF-treated animals.” (p. 1801).
P8526 Randolph,C.L., Bierl,M.A., Isaacson,L.G. Regulation of NGF and NT-3 protein expression in peripheral targets by sympathetic input. Brain Research 2007; 1144(–):59-69. >>> Nerve growth factor, mouse; Bisbenzimide; CSF/CNS; Rat; 2002; 2 weeks; Controls received no treatment; peptides; animal info (female, Sprague-Dawley, 3 months old); bisbenzimide (fluorescent marker) added to infusate to monitor cannula placement.
P8426 Hobara,N., Goda,M., Kitamura,Y., Sendou,T., Gomita,Y., Kawasaki,H. Adrenomedullin facilitates reinnervation of phenol-injured perivascular nerves in the rat mesenteric resistance artery. Neuroscience 2007; 144(-2-):721-730. >>> Adrenomedullin, recomb. human; nerve growth factor; Saline, sterile; IP; Rat; 2001; 7 days; Controls received mp w/ vehicle; dose-response (fig. 2); peptides; animal info (Wistar, 8 wk old, perivascular denervation).
P8023 Niewiadomska,G., Baksalerska-Pazera,M., Gasiorowska,A., Mietelska,A. Nerve growth factor differentially affects spatial and recognition memory in aged rats. Neurochemical Research 2006; 31(-12-):1481-1490. >>> Nerve growth factor, beta; CSF, artificial; CSF/CNS; Rat; 2004; 1 month; ALZET brain infusion kit used; post op. care (antibiotic and analgesic to scalp + fluid therapy); silastic tubing used; 28 G cannula.
P7978 Engelhardt,M., Di Cristo,G., Berardi,N., Maffei,L., Wahle,P. Differential effects of NT-4, NGF and BDNF on development of neurochemical architecture and cell size regulation in rat visual cortex during the critical period. European Journal of Neuroscience 2007; 25(-2-):529-540. >>> Nerve growth factor, mouse; NT-4, human recomb.; brain-derived neurotrophic factor, recomb. human; CSF/CNS (visual cortex); Rat; 1007D; 8 days; Post op. care (antibiotics and local anesthetics); animal info (Long-Evans); dental cement used; 30 G cannula used; tissue perfusion (visual cortex).
P7728 Frielingsdorf,H., Thal,L.J., Pizzo,D.P. The septohippocampal cholinergic system and spatial working memory in the Morris water maze. Behavioural Brain Research 2006; 168(-1-):37-46. >>> Nerve growth factor, recomb. human; CSF, artificial; gentamicin; albumin, rat serum; CSF/CNS; Rat; 2004; 4 weeks; Controls received mp w/ vehicle; functionality of mp verified by residual volume; cannula placement confirmed by cresyl violet staining; peptides; animal info (male, Fisher 344, 13-14 wk. old, medial septum lesion, 400g); mp primed 2 days in 37 Celsius saline.
P7582 Cardoso,A., Paula-Barbosa,M.M., Lukoyanov,N.V. Reduced density of neuropeptide Y neurons in the somatosensory cortex of old male and female rats: Relation to cholinergic depletion and recovery after nerve growth factor treatment. Neuroscience 2006; 137(-3-):937-948. >>> Dye, methylene blue; nerve growth factor; CSF, artificial; BSA; CSF/CNS; Rat; 2002; 14 days; Controls received mp w/ vehicle or sham surgery; functionality of mp verified by pretesting; Alzet brain infusion kit used; post op. care (antiseptic); animal info (male, female, Wistar 6 month old, 24 month old); Lynch coil filled with NGF, air-oil spacer between mp and catheter, mp filled with dye.

6. Placental Growth Factor
Q0490 Takeda,Y., Uemura,S., Iwama,H., Imagawa,K.I., Nishida,T., Onoue,K., Takemoto,Y., Soeda,T., Okayama,S., Somekawa,S., Ishigami,K.I., Takaoka,M., Kawata,H., Kubo,A., Horii,M., Nakajima,T., Saito,Y. Treatment With Recombinant Placental Growth Factor (PIGF) Enhances Both Angiogenesis and Arteriogenesis and Improves Survival After Myocardial Infarction. CIRCULATION JOURNAL 2009; 73(-9-):1674-1682. >>> Placental growth factor, recomb. human; flt-1, recomb. human, soluble; IP; Mice; 3, 7 days; Controls received mp w/ vehicle; animal info (C57BL/6, 12 wks old); polyethylene IP catheter used.
P6669 Tamarat,R., Silvestre,J.S., Ricousse-Roussanne,S., Barateau,V., Lecomte-Raclet,L., Clergue,M., Duriez,M., Tobelem,G., Levy,B.I. Impairment in ischemia-induced neovascularization in diabetes – Bone marrow mononuclear cell dysfunction and therapeutic potential of placenta growth factor treatment. American Journal of Pathology 2004; 164(-2-):457-466. >>> Placental growth factor; SC; Mice; 2001; 14 days; Diabetes, placenta growth factor (PIGF) is a VEGF homologue; ischemia.

7. Transforming Growth Factor
Q2327 Chim,H., Miller,E., Gliniak,C., Alsberg,E. Stromal-cell-derived factor (SDF) 1-alpha in combination with BMP-2 and TGF-beta1 induces site-directed cell homing and osteogenic and chondrogenic differentiation for tissue engineering without the requirement for cell seeding. Cell and Tissue Research 2012; 350(-1-):89-94. >>> Stromal-cell-derived factor-1, alpha; bone morphogenetic protein 2; transforming growth factor-1, beta; IP (abdominal wall); Rat; 2004; 4 weeks; Negative control animals received no cytokines; animal info (Sprague Dawley, adult); “A custom-made apparatus for the constant delivery of cytokines was assembled consisting in a microneedle system and Alzet osmotic pump” pg 90; fig 1b, image of custom-made cytokine delivery apparatus; tissue perfusion (anterior abdominal wall).
Q2324 Holmberg,C., Quante,M., Steele,I., Kumar,J.D., Balabanova,S., Duval,C., Czepan,M., Rakonczay,Z.Jr, Tiszlavicz,L., Nemeth,I., Lazar,G., Simonka,Z., Jenkins,R., Hegyi,P., Wang,T.C., Dockray,G.J., Varro,A. Release of TGF beta ig-h3 by gastric myofibroblasts slows tumor growth and is decreased with cancer progression. Carcinogenesis 2012; 33(-8-):1553-1562. >>> Transforming growth factor-beta-induced gene h3; Mice; ; Animal info (SCID, 6-8 wks old); cancer.
Q1643 Kandasamy,M., Couillard-Despres,S., Raber,K.A., Stephan,M., Lehner,B., Winner,B., Kohl,Z., Rivera,F.J., Nguyen,H.P., Riess,O., Bogdahn,U., Winkler,J., von Hoersten,S., Aigner,L. Stem Cell Quiescence in the Hippocampal Neurogenic Niche Is Associated With Elevated Transforming Growth Factor-beta Signaling in an Animal Model of Huntington Disease. Journal of Neuropathology and Experimental Neurology 2010; 69(-7-):717-728. >>> Transforming growth factor, beta 1, recomb.; CSF, artificial; CSF/CNS; Rat; 2002; 14 days; Controls received mp w/ vehicle; animal info (Fischer 344, female, 2-3 mo old, 180 g).
Q1047 Echeverry,S., Shi,X.Q., Rivest,S., Zhang,J. Peripheral Nerve Injury Alters Blood-Spinal Cord Barrier Functional and Molecular Integrity through a Selective Inflammatory Pathway. Journal of Neuroscience 2011; 31(-30-):10819-10828. >>> Minocycline hydrochloride; MCP-1, recomb., rat; antibody, MCP-1 neutralizing; IL-10, recomb.; transforming growth factor, beta-1; Saline, sterile, isotonic; CSF/CNS (intrathecal); Rat; 2001; 3, 7 days; Controls received mp w/ vehicle; animal info (male, Sprague Dawley, 250-275 g, naive, nerve-injured).
Q0790 Leker,R.R., Toth,Z.E., Shahar,T., Cassiani-Ingoni,R., Szalayova,I., Key,S., Bratincsak,A., Mezey,E. TRANSFORMING GROWTH FACTOR alpha INDUCES ANGIOGENESIS AND NEUROGENESIS FOLLOWING STROKE. Neuroscience 2009; 163(-1-):233-243. >>> Transforming growth factor, alpha; CSF, artificial; CSF/CNS (infarct border); Mice; 1002; 14 days; Controls received mp w/ vehicle; animal info (C57B, male, 4-6 wks old).
P9873 Echeverry,S., Shi,X.Q., Haw,A., Liu,H., Zhang,Z., Zhang,J. Transforming growth factor-beta-1 impairs neuropathic pain through pleiotropic effects. Molecular Pain 2009; 5(-;-):U1-U18. >>> Transforming growth factor-1, beta; CSF/CNS (intrathecal); Rat; 1007D; 2002; 7, 14 days; Animal info (male, Sprague Dawley, 250-275 g); incorrectly stated pump model as 2002D.
P9848 McGrath,L.J., Ingman,W.V., Robker,R.L., Robertson,S.A. Exogenous transforming growth factor beta1 replacement and fertility in male Tgfb1 null mutant mice. REPRODUCTION FERTILITY AND DEVELOPMENT 2009; 21(-4-):561-570. >>> Transforming growth factor beta-1, recomb. human; BSA; PBS; SC; Mice; 1002; 2 weeks; Controls received mp w/ vehicle; comparios of PO gavage vs. mp; half-life (p. 563) 1 hour; animal info (Tgfb1 null); “continuous supply of rhLTGFB1 by subcutaneous insertion of osmotic pumps successfully increased circulating TGFB1 to detectable levels” pg. 568.
P9807 Guerra-Crespo,M., Gleason,D., Sistos,A., Toosky,T., Solaroglu,I., Zhang,J.H., Bryant,P.J., Fallon,J.H. TRANSFORMING GROWTH FACTOR-ALPHA INDUCES NEUROGENESIS AND BEHAVIORAL IMPROVEMENT IN A CHRONIC STROKE MODEL. Neuroscience 2009; 160(-2-):470-483. >>> Transforming growth factor; CSF/CNS; Rat; 2004; 28 days; Controls received mp w/ PBS; ALZET brain infusion kit used; animal info (90 days old, MCAO); behavioral testing (cylinder corner test).
P9772 Cooper,O., Isacson,O. Intrastriatal Transforming Growth Factor Delivery to a Model of Parkinson’s Disease Induces Proliferation and Migration of Endogenous Adult Neural Progenitor Cells without Differentiation into Dopaminergic Neurons. The Journal of Neuroscience 2004; 24(-41-):8924-8931. >>> Transforming growth factor, alpha; PBS; CSF/CNS (striatum); Rat; 2004; 1, 2, 4 weeks; Controls received mp w/ vehicle; ALZET brain infusion kit 2 used; animal info (naive, adult, male, Sprague Dawley); neurodegenerative (Parkinson’s Disease); brain tissue distribution; tissue perfusion (striatum).
P9532 Dufour,C., Holy,X., Marie,P.J. Transforming growth factor-beta prevents osteoblast apoptosis induced by skeletal unloading via PI3K/Akt, Bcl-2, and phospho-Bad signaling. J Physiol Endocrinol Metab 2008; (-294-):E794-E801. >>> Transforming growth factor-beta 2; Rat; 2, 4, 7 days; Animal info (adult, 4 wks old, Wistar, 130g.).
P9402 de Chevigny,A., Cooper,O., Vinuela,A., Reske-Nielsen,C., Lagace,D.C., Eisch,A.J., Isacson,O. Fate mapping and lineage analyses demonstrate the production of a large number of striatal neuroblasts after transforming growth factor alpha and noggin striatal infusions into the dopamine-depleted striatum. Stem Cells 2008; 26(-9-):2349-2360. >>> Transforming growth factor-a; noggin; PBS; CSF/CNS (striatum); Rat; 1002; 2004; 14, 28, 41 days; Controls received mp w/ vehicle; ALZET brain infusion kit 2 used; brain tissue distribution; peptides; animal info (female, Sprague Dawley, 250-300 g.); behavioral testing (rotation behavior); noggin is a bone morphogenetic protein antagonist; neurodegenerative (Parkinson’s Disease).
P9315 Gleason,D., Fallon,J.H., Guerra,M., Liu,J.C., Bryant,P.J. Ependymal stem cells divide asymmetrically and transfer progeny into the subventricular zone when activated by injury. Neuroscience 2008; 156(-1-):81-88. >>> Transforming growth factor-a; PBS; CSF/CNS (caudate putamen); Rat; 1, 3, 5, 7, 28 days; Peptides, animal info (male, 10 wks old); neurodegenerative (Parkinson’s Disease); neural stem cell research.
P9186 White,R.E., Yin,F.Q., Jakeman,L.B. TGF-alpha increases astrocyte invasion and promotes axonal growth into the lesion following spinal cord injury in mice. Experimental Neurology 2008; 214(-1-):10-24. >>> Transforming growth factor-alpha; Serum, mouse; PBS; CSF/CNS (intrathecal); Mice; 2002; 14 days; Controls received mp w/ vehicle; functionality of mp verified by residual volume; post op. care (Baytril); animal info (female, adult, C57BL/6, 10 wks old, 17-20 g.); spinal cord injury; pumps were primed in saline at 37 degree Celsius for 24 hours; pumps were weighed before and after filling; good methods; PE-50 used for catheter.
P8988 Wachs,F.P., Winner,B., Couillard-Despres,S., Schiller,T., Aigner,R., Winkler,J., Bogdahn,U., Aigner,L. Transforming growth factor-beta 1 is a negative modulator of adult neurogenesis. Journal of Neuropathology and Experimental Neurology 2006; 65(-4-):358-370. >>> Transforming growth factor-B1, recomb.; CSF, artificial; CSF/CNS; Rat; 2002; 7 days; Controls received mp w/ vehicle; peptides; animal info (female, Fischer-344, 2-3 months old, 180 g.).

8. Vascular Endothelial Growth Factor
Q2611 Siddiqui,A.H., Irani,R.A., Zhang,W.R., Wang,W., Blackwell,S.C., Kellems,R.E., Xia,Y. Angiotensin Receptor Agonistic Autoantibody-Mediated Soluble Fms-Like Tyrosine Kinase-1 Induction Contributes to Impaired Adrenal Vasculature and Decreased Aldosterone Production in Preeclampsia. Hypertension 2013; 61(-2-):472-U526. >>> Vascular endothelial growth factor, 121; Mice (pregnant); 5 days; Animal info (C57BL/6).
Q2593 Shan,L., Yong,H.M., Song,Q., Wei,Y., Qin,R., Zhang,G.H., Xu,M.Y., Zhang,S.H. Vascular endothelial growth factor B prevents the shift in the ocular dominance distribution of visual cortical neurons in monocularly deprived rats. Experimental Eye Research 2013; 109(-;-):17-21. >>> Vascular endothelial growth factor B; CSF, artificial; CSF/CNS; Rat; 1007D; ; Control animals received mp w/ vehicle; animal info (Long Evans hooded); PE60 tubing used.
Q2468 Xiao,X.W., Guo,P., Chen,Z., El-Gohary,Y., Wiersch,J., Gaffar,I., Prasadan,K., Shiota,C., Gittes,G.K. Hypoglycemia Reduces Vascular Endothelial Growth Factor A Production by Pancreatic Beta Cells as a Regulator of Beta Cell Mass. Journal of Biological Chemistry 2013; 288(-12-):8636-8646. >>> Vascular endothelial growth factor, A; Mice; 2004; ; Animal info (C57BL/6, MIP-GFP, male, 8 wks old);.
Q2316 Argandona,E.G., Bengoetxea,H., Bulnes,S., Rico-Barrio,I., Ortuzar,N., Lafuente,J.V. Effect of intracortical vascular endothelial growth factor infusion and blockade during the critical period in the rat visual cortex. Brain Research 2012; 1473(-;-):141-154. >>> Vascular endothelial growth factor; anti-VEGF; PBS; CSF/CNS (cortex); Rat; 1007D; ; Control animals received mp w/ vehicle; animal info (Long Evans, P18); ALZET brain infusion kit used; cyanoacrylate adhesive used.
Q2201 Mateus,J., Bytautiene,E., Lu,F.X., Tamayo,E.H., Betancourt,A., Hankins,G.D.V., Longo,M., Saade,G.R. Endothelial growth factor therapy improves preeclampsia-like manifestations in a murine model induced by overexpression of sVEGFR-1. American Journal of Physiology-Heart and Circulatory Physiology 2011; 301(-5-):H1781-H1787. >>> Vascular endothelial growth factor 121; PBS; SC; Mice (pregnant); 2002; 10 days; Controls received mp w/ vehicle; animal info (CD-1); functionality of mp verified by residual volume and plasma drug levels.
Q1819 Thau-Zuchman,O., Shohami,E., Alexandrovich,A.G., Leker,R.R. SUBACUTE TREATMENT WITH VASCULAR ENDOTHELIAL GROWTH FACTOR AFTER TRAUMATIC BRAIN INJURY INCREASES ANGIOGENESIS AND GLIOGENESIS. Neuroscience 2012; 202(-;-):334-341. >>> Vascular endothelial growth factor; Saline; CSF/CNS; Mice; 7 days; Controls received mp w/ vehicle; animal info (adult, Sabra, 40 g).
Q1810 Herz,J., Reitmeir,R., Hagen,S.I., Reinboth,B.S., Guo,Z.Y., Zechariah,A., ElAli,A., Doeppner,T.R., Bacigaluppi,M., Pluchino,S., Kilic,U., Kilic,E., Hermann,D.M. Intracerebroventricularly delivered VEGF promotes contralesional corticorubral plasticity after focal cerebral ischemia via mechanisms involving anti-inflammatory actions. NEUROBIOLOGY OF DISEASE 2012; 45(-3-):1077-1085. >>> Vascular endothelial growth factor, recomb. human; CSF/CNS; Mice; 2002; 2004; 4 weeks; Controls received mp w/ saline; animal info (male, C57BL/6J, 8-10 wks old, 23-25 g); ischemia.
Q1775 Lutton,C., Young,Y.W., Williams,R., Meedeniya,A.C.B., kay-Sim,A., Goss,B. Combined VEGF and PDGF Treatment Reduces Secondary Degeneration after Spinal Cord Injury. Journal of Neurotrauma 2012; 29(-5-):957-U479. >>> Vascular endothelial growth factor; platelet-derived growth factor; Saline; CSF/CNS (intrathecal); Rat; 2001; 7 days; Controls received mp w/ vehicle; animal info (Wistar, male, adult, 300 g, 20-25 wks old); pump functionality verified in vitro; “catheter was… sutured to the muscle to keep it in place” pg 959.
Q1664 Chu,L.M., Robich,M.P., Lassaletta,A.D., Feng,J., Laham,R.J., Burgess,T., Clements,R.T., Sellke,F.W. Resveratrol supplementation abrogates pro-arteriogenic effects of intramyocardial vascular endothelial growth factor in a hypercholesterolemic swine model of chronic ischemia. Surgery 2011; 150(-3-):390-399. >>> Vascular endothelial growth factor, recomb. human; Intrapericardial; Pig; ; Animal info (intact, male, Yorkshire); stress/adverse effects “sudden cardiac death”, pg 393.
Q1655 Thau-Zuchman,O., Shohami,E., Alexandrovich,A.G., Leker,R.R. Combination of Vascular Endothelial and Fibroblast Growth Factor 2 for Induction of Neurogenesis and Angiogenesis after Traumatic Brain Injury. Journal of Molecular Neuroscience 2012; 47(-1-):166-172. >>> Vascular endothelial growth factor; Saline; CSF/CNS; Mice; 1007D; 7 days; Controls received mp w/ vehicle; animal info (male, Sabra, adult, 40 g).
Q1620 Reitmeir,R., Kilic,E., Reinboth,B.S., Guo,Z.Y., ElAli,A., Zechariah,A., Kilic,U., Hermann,D.M. Vascular endothelial growth factor induces contralesional corticobulbar plasticity and functional neurological recovery in the ischemic brain. Acta Neuropathologica 2012; 123(-2-):273-284. >>> Vascular endothelial growth factor; NaCl; CSF/CNS; Mice; 2002; 2004; 4 weeks; Controls received mp w/ vehicle; animal info (male, C57Bl6/j, 8-10 wks old, 23-25 g); ischemia (MCAO); stress/adverse effects, “complications related to pump insertion” pg 274; behavioral testing (Rotarod, grip strength test).
Q1592 Romo,L.B., Tapia,R. VEGF protects spinal motor neurons against chronic excitotoxic degeneration in vivo by activation of PI3-K pathway and inhibition of p38MAPK. Journal of Neurochemistry 2010; 115(-5-):1090-1101. >>> Isoxazolepropionate, alpha amino-3-hydroxy-5-; vascular endothelial growth factor, recomb., 164; SU14980, tyrphostin; LY294002; wortmannin; PD98059; SB203580; PBS; DMSO; CSF/CNS (intrathecal, spinal cord); Rat; 2004; 2, 10, 20 days; Controls received mp w/ vehicle; animal info (Wistar, male, 270-290 g, adult); alpha amino-3-hydroxy-5-isoxazolepropionate also known as AMPA; wound clips used; post op. care, pg 1091 (penicillin); good methods, pg 1091; multiple pumps used (2); multiple intrathecal catheters used; wound clips used; 2% DMSO used; enzyme inhibitor (p38 mitogen-activated protein kinase, p38MAPK).
Q1355 Thau-Zuchman,O., Shohami,E., Alexandrovich,A.G., Leker,R.R. Vascular endothelial growth factor increases neurogenesis after traumatic brain injury. Journal of Cerebral Blood Flow and Metabolism 2010; 30(-5-):1008-1016. >>> Vascular endothelial growth factor; CSF/CNS; Mice; 6, 7 days; Controls received mp w/ vehicle or were sham operated; animal info (adult, Sabra, male, 40 g); infusion rate of 0.5 ul/hr.
Q1347 Siddiqui,A.H., Irani,R.A., Zhang,Y.J., Dai,Y.B., Blackwell,S.C., Ramin,S.M., Kellems,R.E., Xia,Y. Recombinant Vascular Endothelial Growth Factor 121 Attenuates Autoantibody-Induced Features of Pre-eclampsia in Pregnant Mice. American Journal of Hypertension 2011; 24(-5-):606-612. >>> Vascular endothelial growth factor 121; SC; Mice (pregnant); 1007D; 5 days; Animal info (C57BL/6, 8 wks old).
Q1236 Miyasaka,E.A., Raghavan,S., Gilmont,R.R., Mittal,K., Somara,S., Bitar,K.N., Teitelbaum,D.H. In vivo growth of a bioengineered internal anal sphincter: comparison of growth factors for optimization of growth and survival. PEDIATRIC SURGERY INTERNATIONAL 2011; 27(-2-):137-143. >>> Fibroblast growth factor-2; vascular endothelial growth factor-2; platelet-derived growth factor; SC; Mice; 1004; 28 days; Controls received mp with no growth factors; animal info (C57BL/6); good methods, pg 138; tissue perfusion (internal anal sphincter); silicone catheter used; “the osmotic pumps we used completed delivery of the growth factors by 28 days, which would clearly limit the duration of exposure to the growth factor, lessening the risk of malignancy” pg 143.
Q1197 Lee,C., Agoston,D.V. Vascular Endothelial Growth Factor Is Involved in Mediating Increased De Novo Hippocampal Neurogenesis in Response to Traumatic Brain Injury. Journal of Neurotrauma 2010; 27(-3-):541-553. >>> Vascular endothelial growth factor 164; SU5416; uridine, bromodeoxy; CSF, artificial; DMSO; CSF/CNS; Rat; 2002; 13 days; Controls received mp w/ vehicle; animal info (young, Sprague Dawley, male, 270-300 g); 1% DMSO used.
Q0878 Ortuzar,N., Argandona,E.G., Bengoetxea,H., Lafuente,J.V. Combination of intracortically administered VEGF and environmental enrichment enhances brain protection in developing rats. Journal of Neural Transmission 2011; 118(-1-):135-144. >>> Vascular endothelial growth factor; CSF/CNS (cortex); Rat; 1007D; 7 days; Controls received mp w/ PBS or no implantation; animal info (Long-Evans, P18); cyanoacrylate adhesive; ALZET brain infusion kit 3 used; “Total operating time was approximately 25 min.” pg 137.
Q0712 Nicoletti,J.N., Lenzer,J., Salerni,E.A., Shah,S.K., Elkady,A., Khalid,S., Quinteros,D., Rotella,F., Betancourth,D., Croll,S.D. Vascular endothelial growth factor attenuates status epilepticus-induced behavioral impairments in rats. EPILEPSY&BEHAVIOR 2010; 19(-3-):272-277. >>> Vascular endothelial growth factor, 165, recomb. human; vascular endothelial growth factor, inactivated; PBS, sterile; CSF/CNS (hippocampus); Rat; 2002; 2 weeks; Controls received no surgical manipulations or protein infusions; animal info (male Sprague-Dawley, 250-350 g); behavioral testing (Morris water maze, Grid locomotor activity, Light-dark exploration).
Q0514 Lee,C., Agoston,D.V. Inhibition of VEGF receptor 2 increased cell death of dentate hilar neurons after traumatic brain injury. Experimental Neurology 2009; 220(-2-):400-403. >>> Vascular endothelial growth factor (164); SU5416; CSF, artificial; DMSO; CSF/CNS; Rat; 1002; 13 days; Controls received mp w/ vehicle; animal info (young, male, Sprague Dawley, 270-330 g.); cannula placement verified by histology; 1% DMSO used; artificial CSF recipe.
Q0400 Bogaert,E., Van Damme,P., Poesen,K., Dhondt,J., Hersmus,N., Kiraly,D., Scheveneels,W., Robberecht,W., Van den Bosch,L. VEGF protects motor neurons against excitotoxicity by upregulation of GluR2. NEUROBIOLOGY OF AGING 2010; 31(-12-):2185-2191. >>> Vascular endothelial growth factor; CSF/CNS; Rat; 2004; 1 week; Controls received mp w/ vehicle; animal info (80 days old).
Q0354 Ahmed,A., Singh,J., Khan,Y., Seshan,S.V., Girardi,G. A New Mouse Model to Explore Therapies for Preeclampsia. PLoS One 2010; 5(-10-):U325-U333. >>> Vascular endothelial growth factor (121); PBS, sterile; SC; Mice; 7 days; Animal info (CBA/J x DBA/2 and CBA/J x BALB/c).
Q0282 Schmidt,C., Bezuidenhout,D., Beck,M., Van der Merwe,E., Zilla,P., Davies,N. Rapid three-dimensional quantification of VEGF-induced scaffold neovascularisation by microcomputed tomography. Biomaterials 2009; 30(-30-):5959-5968. >>> Vascular endothelial growth factor (165); SC; Rat; 1002; 10 days; Controls received mp w/ PBS; animal info (male, Wistar, 234-254 g); functionality of mp verified by residual volume; post op. care (buprenorphine); pump connected to a porous polyurethane construct, schematic on Figure 1A;.
P9814 Santulli,G., Ciccarelli,M., Palumbo,G., Campanile,A., Galasso,G., Ziaco,B., Altobelli,G.G., Cimini,V., Piscione,F., D’Andrea,L.D., Pedone,C., Trimarco,B., Iaccarino,G. In vivo properties of the proangiogenic peptide QK. Journal of Translational Medicine 2009; 7(-;-):U1-U10. >>> Vascular endothelial growth factor-15; vascular endothelial growth factor-165; QK; IA (femoral); Rat; 2002; 14 days; Peptides; animal info (12 wks old, WKY, normosensitive); QK is a de novo engineered VEGF mimicking peptide.
P9708 Schmidt,N.O., Koeder,D., Messing,M., Mueller,F.J., Aboody,K.S., Kim,S.U., Black,P.M., Carroll,R.S., Westphal,M., Lamszus,K. Vascular endothelial growth factor-stimulated cerebral microvascular endothelial cells mediate the recruitment of neural stem cells to the neurovascular niche. Brain Research 2009; 1268(-;-):24-37. >>> Vascular endothelial growth factor, recomb. human; CSF/CNS (parenchyma); Mice (nude); 2004; ; Controls received mp w/PBS; animal info (6 wks old).
P9443 Segi-Nishida,E., Warner-Schmidt,J.L., Duman,R.S. Electroconvulsive seizure and VEGF increase the proliferation of neural stem-like cells in rat hippocampus. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2008; 105(-32-):11352-11357. >>> Vascular endothelial growth factor 164, recomb. rat; PBS; CSF/CNS; Rat; 1003D; 3 days; 24 hours; Controls received mp w/ vehicle; brain tissue distribution; peptides; animal info (male, Sprague Dawley, 175-200 g.); delayed delivery (in the 48 hour infusion, PBS infused for 24 hours to “avoid the influence of cannulation and anesthesia.” (p. 11354) then VEGF infused for 24 hours).
P9248 Poesen,K., Lambrechts,D., Van Damme,P., Dhondt,J., Bender,F., Frank,N., Bogaert,E., Claes,B., Heylen,L., Verheyen,A., Raes,K., Tjwa,M., Eriksson,U., Shibuya,M., Nuydens,R., Van den Bosch,L., Meert,T., D’Hooge,R., Sendtner,M., Robberecht,W., Carmeliet,P. Novel Role for Vascular Endothelial Growth Factor (VEGF) Receptor-1 and Its Ligand VEGF-B in Motor Neuron Degeneration. Journal of Neuroscience 2008; 28(-42-):10451-10459. >>> Vascular endothelial growth factor-B186, recomb. mouse; CSF, artificial; CSF/CNS; Rat; 2004; 100 days; Controls received mp w/ vehicle; long-term study; pumps replaced every 25 days; no stress (see pg. 10456); stability verified by 4 weeks in mp at 37 degrees Celsius; animal info (HAN-Wistar x Sprague Dawley-hSOD1G93A, 60 days old); behavioral testing (Rotarod test); “this delivery route was chosen because previous studies demonstrated that intracerebroventricularly delivered VEGF diffuses from the CSF into the neural parenchyma, where it reaches it’s target motor neurons.” (p. 10456).
P9198 Rennel,E.S., Hamdollah-Zadeh,M.A., Wheatley,E.R., Magnussen,A., Schueler,Y., Kelly,S.P., Finucane,C., Ellison,D., Cebe-Suarez,S., Ballmer-Hofer,K., Mather,S., Stewart,L., Bates,D.O., Harper,S.J. Recombinant human VEGF165b protein is an effective anti-cancer agent in mice. European Journal of Cancer 2008; 44(-13-):1883-1894. >>> Vascular endothelial growth factor-165-b, recomb.; NaCl, sterile; SC; Mice; 28 days; No stress (see pg. 1888); half-life (p. 1893) 25 min in mice, 13.8-72 min in rats; cancer (colon); post op. care (Temgesic); animal info (C57/Bl6, 720 g.); silk sutures used; “no adverse effects were observed with this infusion of VEGF165b” pg. 1888; compound is an anti angiogenic isoform of VEGF.
P9139 Nicoletti,J.N., Shah,S.K., McCloskey,D.P., Goodman,J.H., Elkady,A., Atassi,H., Hylton,D., Rudge,J.S., Scharfman,H.E., Croll,S.D. Vascular endothelial growth factor is up-regulated after status epilepticus and protects against seizure-induced neuronal loss in hippocampus. Neuroscience 2008; 151(-1-):232-241. >>> Vascular endothelial growth factor, recomb. human; BowAng1; Flt-Fc; CSF/CNS (dorsal hippocampus); Rat; 5 days; Controls received mp w/ PBS; animal info (male, Sprague Dawley, adult, 250-350 g.); cannula, polyvinyl catheter from Plastics One used; BowAng1 is a fusion of four molecules of angiopoietin-1 with two molecules of hFC; Flt-Fc is an immunoadhesin.
P9097 Ohm,J.E., Gabrilovich,D.I., Sempowski,G.D., Kisseleva,E., Parman,K.S., Nadaf,S., Carbone,D.P. VEGF inhibits T-cell development and may contribute to tumor-induced immune suppression. Blood 2003/6/15; 101(-12-):4878-4886. >>> Vascular endothelial growth factor; SC; Mice (transgenic); 7-28 days; Controls received mp w/ PBS; no stress (see pg. 4880); animal info (female, 6-8 wks old, DO11.10); immunology.
P8833 Boodhwani,M., Voisine,P., Rue,M., Sodha,N.R., Feng,J., Xu,S.H., Bianchi,C., Settke,F.W. Comparison of vascular endothelial growth factor and fibroblast growth factor-2 in a swine model of endothelial dysfunction. European Journal of Cardio-thoracic Surgery 2008; 33(-4-):645-650. >>> Vascular endothelial growth factor 165, recomb. human; Heparin; Intramyocardial; Pig (miniswine); 2ML4; 4 weeks; Tissue perfusion (myocardial); half-life (p. 649) “short”; cardiovascular; peptides; ischemia (cardiac); animal info (Yucatan mini-swine, 20-30 kg.).
P8799 Tsuchiya,A., Heike,T., Baba,S., Fujino,H., Umeda,K., Matsuda,Y., Nomoto,M., Ichida,T., Aoyagi,Y., Nakahata,T. Sca-1+ endothelial cells (SPECs) reside in the portal area of the liver and contribute to rapid recovery from acute liver disease. Biochemical and Biophysical Research Communications 2008; 365(-3-):595-601. >>> Vascular endothelial growth factor-inhibitor; DMSO; IP; Mice; 9, 11 days; Controls received mp w/ vehicle; animal info (C57BL/6; 4, 8, 12 wks old; anti-Fas Ab-induced liver damage); agent also known as CBO-P11.
P8512 Huang,Y., Chen,X., Dikov,M.M., Novitskiy,S.V., Mosse,C.A., Yang,L., Carbone,D.P. Distinct roles of VEGFR-1 and VEGFR-2 in the aberrant hematopoiesis associated with elevated levels of VEGF. Blood 2007; 110(-2-):624-. >>> Vascular endothelial growth factor 165, recomb. human; vascular endothelial growth factor, receptor-2 selective; vascular endothelial growth factor, receptor-1 selective; Mice; 2004; 28 days; Controls received mp w/ PBS; cancer; peptides; animal info (female, Balb/c, 6-8 weeks old); angiogenesis; hematology.
P8366 Dorrell,M.I., Aguilar,E., Scheppke,L., Barnett,F.H., Friedlander,M. Combination angiostatic therapy completely inhibits ocular and tumor angiogenesis. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2007; 104(-3-):967-972. >>> Vascular endothelial growth factor, aptamer; EMD472523; T2-TrpRS; PBS; CSF/CNS (intratumoral); Rat; 2001D; 2ML2; 2ML4; 3,6 days; 24 hours; Controls received mp w/ vehicle; cancer (gliosarcoma); animal info (male, Fischer, 344); EMD472523 is an integrin antagonist, VEGF aptamer is chemically identical to Macugen; T2 is a proteolytic fragment of tryptophan tRNA synthetase; all agents have angiostatic activity; tissue perfusion (tumor).
P8306 Tiranathanagul,K., Dhawan,V., Lytle,I.F., Zhang,W., Borschel,G.H., Buffington,D.A., Tziampazis,E., Brown,D.L., Humes,H.D. Tissue engineering of an implantable bioartificial hemofilter. ASAIO Journal 2007; 53(-2-):176-186. >>> Platelet-derived growth factor-BB; vascular endothelial growth factor 164; Saline, normal; albumin, rat serum; acetic acid; IA (femoral); IV (femoral); Rat; 2004; 28 days; Artificial kidney; controls received mp w/ vehicle; peptides; animal info (male, Fisher 344); mp attached by cannula to bioartificial hemofilter device, which surrounded and delivered agent to the femoral vessels; nephrology.
P8299 Cruze,C.A., Su,F., Limberg,B.J., Deutsch,A.J., Stoffolano,P.J., Dai,H.J., Buchanan,D.D., Yang,H.T., Terjung,R.L., Spruell,R.D., Mittelstadt,S.W., Rosenbaum,J.S. The Y2 receptor mediates increases in collateral-dependent blood flow in a model of peripheral arterial insufficiency. Peptides 2007; 28(-2-):269-280. >>> Peptide YY, recomb. human; peptide YY (3-36), recomb. human; vascular endothelial growth factor 165; PBS; glycerol; sodium citrate; Tween 20; IA (iliac); Rat; 14 days; Controls received mp w/ vehicle; replacement therapy (femoral artery occlusion); dose-response (fig. 4); half-lfie (p. 276)<30 minutes; cardiovascular; peptides; ischemia (hindlimb); animal info (male, Sprague-Dawley, 325-350 grams).
P8267 Warner-Schmidt,J.L., Duman,R.S. VEGF is an essential mediator of the neurogenic and behavioral actions of antidepressants. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2007; 104(-11-):4647-4652. >>> Vascular endothelial growth Factor 164, recomb. rat; CSF/CNS; Rat; 1003D; 3 days; Controls received mp w/ vehicle; peptides; animal info (male, Sprague-Dawley, 175-200g.).
P8163 Boodhwani,M., Mieno,S., Voisine,P., Feng,J., Sodha,N., Li,J., Sellke,F.W. High-dose atorvastatin is associated with impaired myocardial angiogenesis in response to vascular endothelial growth factor in hypercholesterolemic swine. Journal of Thoracic and Cardiovascular Surgery 2006; 132(-6-):1299-1306. >>> Vascular endothelial growth factor 165, recomb. human; Heparin; Perivascular (circumflex coronary artery); Mini-pig; 2ML4; 4 weeks; Cardiovascular; peptides; ischemia (cardiac); animal info (male, female, yucatan, 20-30 kg, 23 weeks old).
P8096 Gosain,A., Matthies,A.M., Dovi,J.V., Barbul,A., Gamelli,R.L., DiPietro,L.A. Exogenous pro-angiogenic stimuli cannot prevent physiologic vessel regression. Journal of Surgical Research 2006; 135(-2-):218-225. >>> Vascular endothelial growth factor 164, recomb.; platelet-dreived growth factor; fibroblast growth factor-2; Wound site; Mice; 2002; 11 days; Controls received mp w/ PBS; functionality of mp verified by VEGF levels in wound sponges, residual volume; stability verified by activity of residual VEGF in endothelial cell cord formation assay (fig.4); cardiovascular; peptides; animal info (female, BALB/c, 8-9 wks old, implanted sponge wounds); “The activity of VEGF isolated from the pump was comparable to fresh recombinant VEGF 164, confriming that the recombinant growth factors present in the mini-osmotic pump retain robust biological activity.” (p.221).
P8017 Kawai,T., Takagi,N., Mochizuki,N., Besshoh,S., Sakanishi,K., Nakahara,M., Takeo,S. Inhibitor of vascular endothelial growth factor receptor tyrosine kinase attenuates cellular proliferation and differentiation to mature neurons in the hippocampal dentate gyrus after transient forebrain ischemia in the adult rat. Neuroscience 2006; 141(-3-):1209-1216. >>> Vascular endothelial growth factor receptor tyrosine kinase inhibitor; DMSO; CSF/CNS (dentate gyrus); Rat; 2001; 7 days; Controls received mp w/ vehicle; dose-response (fig. 4); enzyme inhibitor (VEGF receptor tyrosine kinase); ischemia (transient forebrain); animal info (male, Wistar, 8 weeks old, carotid artery occlusion); 0.5% DMSO; agent also known as 4-[(4′-chloro-2′-fluoro) phenylamino]-6,7-dimethoxyquinazoline; tissue perfusion (dentate gyrus).
P7987 Wang,Y.M., Galvan,V., Gorostiza,O., Ataie,M., Jin,K.L., Greenberg,D.A. Vascular endothelial growth factor improves recovery of sensorimotor and cognitive deficits after focal cerebral ischemia in the rat. Brain Research 2006; 1115(–):186-193. >>> Vascular endothelial growth factor; CSF, artificial; CSF/CNS; Rat; 1003D; 3 days; Controls received mp w/ vehicle; ischemia (cerebral); behavioral study.
P7784 Gaudio,E., Barbaro,B., Alvaro,D., Glaser,S., Francis,H., Ueno,Y., Meininger,C.J., Franchitto,A., Onori,P., Marzioni,M., Taffetani,S., Fava,G., Stoica,G., Venter,J., Reichenbach,R., De Morrow,S., Summers,R., Alpini,G. Vascular endothelial growth factor stimulates rat cholangiocyte proliferation via an autocrine mechanism. Gastroenterology 2006; 130(-4-):1270-1282. >>> Vascular endothelial growth factor-A, recomb. mouse; vascular endothelial growth factor-C, recomb. mouse; Saline; BSA; IP; Rat; 1 week; Controls received mp w/ vehicle; peptides; animal info (male, Fischer 344, 150-175g.).
P7731 Carr,A.N., Howard,B.W., Yang,H.T., Eby-Wilkens,E., Loos,P., Varbanov,A., Qu,A., DeMuth,J.P., Davis,M.G., Proia,A., Terjung,R.L., Peters,K.G. Efficacy of systemic administration of SDF-1 in a model of vascular insufficiency: Support for an endothelium-dependent mechanism. Cardiovascular Research 2006; 69(-4-):925-935. >>> Vascular endothelial growth factor; stromal cell-derived factor-1a; PBS; glycerol; sodium acetate; sodium azide; IA (iliac); Rat; 2 weeks; Controls received mp w/ vehicle; dose-response (table 1); cardiovascular; peptides; animal info (adult); bilateral femoral artery ligation; occlusive cardiovascular disease.
P7561 Sun,Y.J., Jin,K.L., Childs,J.T., Xie,L., Mao,X.O., Greenberg,D.A. Vascular endothelial growth factor-B (VEGFB) stimulates neurogenesis: Evidence from knockout mice and growth factor administration. Developmental Biology 2006; 289(-2-):329-335. >>> Vascular endothelial growth factor-B; CSF, artificial; CSF/CNS; Rat; mice; 1003D; 3 days; Controls received mp w/ vehicle; ischemia (cerebral); animal info (mice, 10-12 wk old; male, Sprague-Dawley).

Related Publications

Dr. Penn’s Publications Listed in PubMed

Mayorga, M.E., Penn, M.S. miR-145 is differentially regulated by TGFβ1 and ischemia and targets disabled-2 expression and wnt/β-catenin activity. J Cell Mol Med, In Press (2011)

Sundararaman, S., Kiedrowski, M., Miller, T., Forudi, F., Pastore, J., Penn, M.S. Optimal SDF-1 gene transfer increases cardiac function in ischemic cardiomyopathy. Gene Therapy (In Press) 2011.

Medicetty, S., Wiktor, D., Lehman, N., Raber, A., Buakhamsri, A., Popović, Z. B., Deans, R. Ting, A.E., Penn, M.S.  Percutaneous adventitial delivery of allogeneic bone marrow derived stem cells via infarct related artery improves long-term ventricular function in acute myocardial infarction.   Cell Transplantation  (In Press) 2011.
Agarwal, U., Zhou, X., Weber, K., Dadabayev, A.R., Penn, M.S. Critical role for white blood cell NADPH oxidase mediated plasminogen activator inhibitor-1 oxidation and ventricular rupture following acute myocardial.  J Mol Cell Cardiol 50:426-32, 2011.
Mayorga, M., Dong, F., Mal, N., Jiang, Y., Howe, P.H., Penn, M.S.  Inverse relationship between paracrine and cell associated effects of cardiovascular stem cell therapy. Stem Cells Dev.  20:681-93, 2011.
Sopko N.A., Turturice B.A., Becker M.E., Brown C.R., Dong F., Popović Z.B., Penn M.S.  Bone marrow support of the heart in pressure overload is lost with aging.  PLoS One 5:e15187, 2010.
Aksoy O., Yousefzai R., Singh D., Agarwal S., O’Brien B., Griffin B.P., Kapadia S.R., Tuzcu M.E., Penn M.S., Nissen S.E., Menon V.  Cardiogenic shock in the setting of severe aortic stenosis: role for intra-aortic balloon pump support.  Heart  (In Press) 2010.

Agarwal, U., Ghalani, W., Weber, K., Zou, Y.R., Rabbany, S.Y., Rafii, S., Penn, M.S.  Role of cardiac myocyte CXCR4 expression in development and left ventricular remodeling after acute myocardial infarction. Circ Res 107:667-76, 2010. (PMC2935208)

Penn, M.S. SDF-1:CXCR4 axis is fundamental for tissue preservation and repair.  Invited Editorial Am J Pathol  177:2166-8, 2010.

Wang K., Zhou X., Huang Y., Khalil M., Wiktor D., van Giezen J.J., Penn M.S.  Adjunctive treatment with ticagrelor, but not clopidogrel, added to tPA enables sustained coronary artery recanalisation with recovery of myocardium perfusion in a canine coronary thrombosis model.  Thromb Haemost 104:609-17, 2010
Willerson J.T., Perin E.C., Ellis S.G., Pepine C.J., Henry T.D., Zhao D.X., Lai D., Penn M.S., Byrne B.J., Silva G., Gee A., Traverse J.H., Hatzopoulos A.K., Forder J.R., Martin D., Kronenberg M., Taylor D.A., Cogle C.R., Baraniuk S., Westbrook L., Sayre S.L., Vojvodic R.W., Gordon D.J., Skarlatos S.I., Moyé L.A., Simari R.D.; Cardiovascular Cell Therapy Research Network (CCTRN).   Intramyocardial injection of autologous bone marrow mononuclear cells for patients with chronic ischemic heart disease and left ventricular dysfunction (First Mononuclear Cells injected in the US [FOCUS]): Rationale and design.  Am Heart J  160:215-23, 2010
Rabbany, S.Y., Pastore, J. Yamamoto, M., Miller, T., Rafii, S., Aras, R., Penn, M.  Continuous delivery of stromal cell-derived factor-1 from alginate scaffolds accelerates wound healing.  Cell Transplantation 19:399-408, 2010.

Dong, F., Khalil, M., Kiedrowski, M. Zhou, X., Penn, M.S. Critical role for leukocyte hypoxia inducible factor-1α expression in post-MI left ventricular remodeling.  Circ Res 106:601-10, 2010.

Penn M.S., Agarwal U. IGF-1 and mechanisms of myocardial repair. Invited Editorial Int J Cardiol 7;138(1):1-2, 2010.
Penn, M.S., Mayorga, M.E. Searching for understanding with the cellular lining of life.  Invited Editorial  Circ. Res. 106:1554-6, 2010. (PMC2753196)
Reviews and Book Chapters

Penn, M.S., Bakken, E.E.  Heart-Brain medicine: update 2009.  Cleve Clin J Med.  77  Suppl 3:S4-6, 2010.

Pozuelo, L., Tesar, G., Zhang, J., Penn, M., Franco, K., Jiang, W. Depression and heart disease: what do we know, and where are we headed.  Cleve Clin J Med. 76:59-70, 2009
Penn, M.S., Bakken, E.E.  Heart-brain medicine: Update 2008. Cleve Clin J Med. 76 Suppl 2:S5-7, 2009.
Mayorga, M., Finan, A., Penn, M. Pre-transplantation Specification of Stem Cells to Cardiac Lineage for Regeneration of Cardiac Tissue.  Stem Cell Rev Rep. 5:51-60, 2009.
Goel SS, Harvey JE, Penn M, Menon V.  Images in cardiovascular medicine. Left anterior descending coronary artery occlusion secondary to blunt chest trauma. Circulation. 119:1975-6, 2009.
Sherman W, Dimmeler S, Hare JM, Penn M. Cardiovascular repair and regeneration 2008: the fourth International Conference on Cell Therapy for Cardiovascular Disease (IC3D).  EuroIntervention 4:47-9, 2008.
Penn, M.S., Bakken, E.E.  Heart-Brain medicine: Update 2008.  Cleve Clin J Med. 76 Suppl 2:S5-7, 2009.
Penn, M.S., Khalil, M. Exploiting stem cell homing for gene delivery.  Expert Opinions in Biotechnology Expert Opin Biol Ther. 8:17-30, 2008.
Shpargel, K.B., Jalabi, W., Jin, Y., Dadabayev, A., Penn, M.S., Trapp, B.D.  Preconditioning paradigms and pathways in the brain.  Cleve Clin J Med. 75 Suppl 2:S77-82, 2008.
Penn, M.S., Mangi, A.A., Genetic Enhancement of Stem Cell Engraftment, Survival and Efficacy.  Circ Res, 102:1471-82, 2008.
Penn, M.S., Bakken, E.E.  Heart-brain medicine: update 2007.  Cleve Clin J Med. 75 Suppl 2:S3-4, 2008.
Penn, M.S., The role of leukocyte-generated oxidants in left ventricular remodeling.  Am J Cardiol. 101:30D-33D, 2008.
Penn, M.S., Bakken, EE.  Heart-brain medicine: where we go from here and why.  Cleve Clin J Med. 74 Suppl 1:S4-6, 2007.
Penn, M.S., Mal, N. Stem cells in cardiovascular disease: methods and protocols.  Methods Mol Med. 2006;129:329-51.
Penn, M.S. Stem-cell therapy after acute myocardial infarction: the focus should be on those at risk. Invited Editorial Lancet. 367:87-8, 2006

Electrical stimulation of muscle progenitor cells – Repository …
repository.tue.nl/posters/738471.pdf 

Electrical stimulation directly induces pre-angiogenic …
jcs.biologists.org/content/117/3/397.full 

Alignment of Skeletal Muscle Cells Cultured in Collagen Gel …
www.hindawi.com/journals/ijte/…/62152… 

Dynamics of Myoblast Transplantation Reveal a Discrete Minority of Precursors

The Partial Regeneration of Lost Limbs with Electrical Stimulation – Dr. Robert O. Becker

Low-intensity Electrical Stimulation in Wound Healing

Electrical stimulation to accelerate wound healing

In Situ Electrostimulation Drives a Regenerative Shift in the Zone of Infarcted Myocardium

 Induction of myogenic differentiation by SDF-1 via CXCR4 and CXCR7 receptors.

Polarity effect of microcurrent electrical stimulation

Electrical Stimulation of Myoblast Proliferation and …
onlinelibrary.wiley.com/doi/10.1002/adhm…/abstract

The stimulation of myoblast differentiation by electrically …
www.ncbi.nlm.nih.gov/…  

Novel electrical stimulation sets the cultured myoblast …
www.ncbi.nlm.nih.gov/…  

Myogenic Differentiation of Mesenchymal Stem Cells in a …
www.hindawi.com/journals/bmri/2013/935046/

[PDF]Electrical stimulation of muscle progenitor cells increases …
repository.tue.nl/posters/738406.pdf

The stimulation of myoblast differentiation by electrically …
www.sciencedirect.com/science/…/S0142961208010508  

Accelerated differentiation of myoblast with electric pulses in …
ieeexplore.ieee.org/…/abs…  

Role of MicroRNAs in Human Skeletal Muscle Tissue …

ADIPOSE STEM CELL DIFFERENTIATION TOWARDS VASCULAR LINEAGES USING NOVEL 3D ELECTRICAL STIMULATION SYSTEM

Presenter: Authors:

Miina Bjorninen, MSC
Bjorninen M, Gilmore K, Pelto J, Kellomaki M, Miettinen S, Wallace G, Grijpma D, Haimi S

BioMediTech University of Tampere

Electrical stimulation (ES) has potential for the directed differentiation of human adipose stem cells (hASCs) towards desired lineages in the presence or absence of chemical induction. To date, reusable in vitro electrical stimulation devices that can stimulate several cell constructs simultaneously are unavailable commercially. We therefore developed a system that could electrically stimulate cells reliably and repeatably. Conductive polypyrrole (PPy) coatings on poly (trimethylene carbonate) (PTMC) scaffolds were tested for smooth muscle cell (SMC) and endothelial cell (EC) differentiation of human ASCs with and without ES.

In the first part of the study PTMC scaffolds were chemically coated with PPy in the presence of hyaluronic acid (HA) and their effects on SMC and EC differentiation of hASCs was studied. In the second part of the study, pulsed biphasic electric current (BEC) was applied through the hASC-seeded PPy-coated constructs using a novel in-house ES electrodes composed of titanium nitride (TiN) coated metallic titanium. BEC was applied under SMC differentiation conditions with pulse widths of 0.25 and 1 ms. Cells were characterized for their attachment, viability, cell number as well as EC and SMC marker expression via immunofluorescence staining.

PPy-coated scaffolds provided stronger cell attachment, induced higher proliferation as well as stronger SMC and EC marker expression for hASCs, compared to uncoated PTMC scaffolds under both differentiation conditions. Cells on both scaffold types showed similar viability. Cells under BEC with 1 ms pulse width showed similar viability to controls whereas with 0.25 ms pulse width viability was reduced after 14 days of BEC. Similarly, cells undergoing BEC with 1 ms pulse width showed similar marker expression as that of controls whereas 0.25 ms pulse width showed lower expression levels.

In conclusion, the novel ES electrodes were found to be suitable for delivering BEC for hASCs and can be recommended for in vitro use due to their stability and reliability with repeated use. PPy coating promoted hASC attachment, proliferation and SMC and EC marker expression. The novel electrical stimulation system therefore has potential for vascular tissue engineering applications.

The CXCR4/SDF1 Axis Improves Muscle Regeneration

Regeneration of chronic myocardial infarction by injectable hydrogels containing stem cell homing factor SDF-1 and angiogenic peptide Ac-SDKP.

The effect of VEGF on the myogenic differentiation of adipose tissue derived stem cells within thermosensitive hydrogel matrices

IGF-1 Overexpression Rescues the Failing Heart – Circulation Research

circres.ahajournals.org/content/90/6/631.full

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Adult, hemizygous IGF–1 mice have enlarged hearts, with a progressive … the rate of cardiomyocyte cell loss and the rate of cardiomyocyte regeneration. Further …

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New insights into IGF-1 signaling in the heart: Trends in Endocrinology …

www.cell.com/trends/endocrinology-metabolism/references/S1043-2760(13)00207-5

The potential effects of IGF–1 and GH on patients with chronic heart failure. ….. and Lee, R.T. Cardiac stem cell therapy and the promise of heart regeneration.

Igf Signaling is Required for Cardiomyocyte Proliferation during … – NCBI

www.ncbi.nlm.nih.gov/pubmed/23840646

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The IGF-1 Receptor Identifies a Pool of Human Cardiac Stem Cells …

www.ncbi.nlm.nih.gov › NCBI › Literature › PubMed Central (PMC)

by D D’Amario – ‎2011 – ‎Related articles

May 5, 2011 – The IGF–1 Receptor Identifies a Pool of Human Cardiac Stem Cells with … The ability of IGF-1R-positive-hCSCs to regenerate infarcted …